HPLC测定番石榴叶中金丝桃苷的含量  

Determination of Hyperin in Psidium Guajava Leaf by HPLC

作　　者：卢平华 钱陈钦 黄晓琼 

机构地区：[1]广州市和藤医药研究开发有限公司,广州510330

出　　处：《中国现代应用药学》2011年第6期553-555,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立番石榴叶中金丝桃苷的HPLC含量测定方法。方法采用Dikma Kromasil C18色谱柱（4.6 mm×250 mm,5μm）,以甲醇-0.1%磷酸溶液（45∶55）为流动相,流速为1.0 mL.min-1,检测波长为254 nm。结果金丝桃苷在0.020 48~0.204 8μg内呈良好的线性关系（r=0.999 9）,平均回收率为98.4%,RSD为1.5%。结论本方法简单、准确、重复性好、专属性强,可用于番石榴叶药材及制剂中金丝桃苷的含量测定。OBJECTIVE To establish a determination method for hyperin in Psidium guajava leaf by HPLC. METHODS HPLC was performed on a Dikma Kromasil C18 column（4.6 mm×250 mm, 5 μm） with methanol-0.1% phosphoric acid solution（45∶55） as the mobile phase at a flow rate of 1.0 mL.min-1, and the detection wavelength was 254 nm. RESULTS It showed a good linearity at the range of 0.020 48~0.204 8 μg（r=0.999 9）, and the average recovery of hyperin was 98.4%（RSD= 1.5%）. CONCLUSION The method is sensitive, accurate, reproducible, specific and could be used to determine hyperin in Psidium guajava leaf.

关 键 词：高效液相色谱法 番石榴叶 金丝桃苷 

分 类 号：R284.1[医药卫生—中药学] R917.101[医药卫生—中医学]