分子印迹技术特异性识别头孢类分子的光谱研究  

Spectroscopy Study on the Selectively Distinguishing Cefalexin with the Molecular Imprinted Polymer

作　　者：蒋宇翔[1] 庞道林[1] 何强[2] 赖承钺[1] 郑怀礼[2] 李方[1] 

机构地区：[1]四川大学化学学院,绿色化学与技术教育部重点实验室,四川成都610064 [2]重庆大学三峡库区生态环境教育部重点实验室,重庆400045

出　　处：《光谱学与光谱分析》2011年第7期1852-1856,共5页Spectroscopy and Spectral Analysis

基　　金：国家水体污染控制与治理科技重大专项项目(2008ZX07315-003)资助

摘　　要：以头孢氨苄（CFL）为特异性分析识别对象,采用本体聚合和悬浮聚合两种聚合方法,制备了头孢氨苄分子印迹聚合物。紫外光谱研究证实模板分子CFL与丙烯酰胺之间形成氢键型配合物。用红外光谱法分析了CFL分子印迹聚合物的聚合程度及聚合物表面上存在的功能基团。紫外光谱分析表明,用AM-EGD-MA体系和本体聚合法制备的印迹聚合物对CFL具有高吸附容量。静态吸附实验和选择性吸附研究表明,所合成的分子印迹聚合物通过氢键作用形成了有效的识别官能团,该分子印迹聚合物对头孢氨苄有很好的选择性识别能力。将这种材料作为固相萃取吸附剂用于极性环境样品中头孢氨苄的萃取富集,回收率达到了99.3%~99.7%。Molecular imprinted polymers of cefalexin（CFL） were prepared by non-covalent molecular imprinting technique in the present paper.Using CFL as template molecule,acrylamide（AM） or methacrylic acid（MAA） as functional monomer,ethylene dimethacrylate（EGDMA） as cross-linker,AIBN as initiator and methanol as porogen agent,different molecularly imprinted polymers（MIPs） of CFL were synthesized by bulk or suspension polymerization as synthetic method.The intermolecular action between AM and CFL was investigated by UV and IR spectrophotometric analysis,and the results indicated that polymerizing functional monomer AM could bond effectively with template molecule CFL.By using UV spectrophotometric analysis method,it was found that the MIP prepared with AM-EGDMA by bulk polymerization showed the highest binding capacity for CFL.Test of selective adsorption made clear that the MIP represented more excellent identification property to CFL than to cefadroxol and ampicillin.The MIPs were used as solid-phase extraction sorbent for extraction and enrichment of CFL in actual samples.And the recoveries for CFL extraction were found to be 99.3%~99.7%（n=3）,demonstrating the feasibility of the prepared MIPs for CFL extraction.

关 键 词：分子印迹聚合物 头孢氨苄 光谱表征 固相萃取 

分 类 号：O657[理学—分析化学]