GAE-AFS法分析藏药当佐中总汞与人工胃液中的游离态汞  

Determination of Total Mercury and Ionic Mercury in Artificial Gastric Juice of Tibetan Medicine Dangzuo by GAE-AFS

作　　者：李岑[1,2,3] 杨红霞[1,2,3] 魏立新[1,2] 杜玉枝[1,2] 张国英[1,2,3] 

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810008 [2]青海省藏药药理学和安全性评价研究重点实验室,青海西宁810008 [3]中国科学院研究生院,北京100049

出　　处：《光谱学与光谱分析》2011年第7期1950-1953,共4页Spectroscopy and Spectral Analysis

基　　金：国家"十一五"科技支撑计划项目(2007BAI48B02)资助

摘　　要：藏药中的重金属汞已成为藏药安全性评价研究的重点。该文以藏药当佐为研究对象,分别采用湿法消解和人工胃液处理,利用金汞齐富集-冷原子荧光法（GAE-AFS）,测定当佐中总汞含量及其在人工胃液中游离态汞含量,并对所建立的方法及仪器的工作条件进行了考察。在选定的测试条件下,仪器系统稳定性良好,HgS的回收率（n=6）为99.56%（RSD=1.94%）,Hg的检出限为0.2 ng.L-1,线性范围为0-500ng.L-1,r=0.999 9。同时,应用该方法对不同地区来源的当佐进行了测定,结果总汞含量变化范围为3.998 0-16.735 8 mg.g-1,游离汞含量变化范围为45.537 7-1 033.985 0 ng.g-1,表明不同来源样品中总汞及其在人工胃液中游离态汞含量具有一定差异。该方法快速、灵敏,适合于中、藏药中的总汞及其在人工胃液中的游离汞的测定。The mercury in Tibetan medicine has become important focus in the research on medicine safety evaluation.The total mercury and the ionic mercury in artificial gastric juice of Tibetan medicine Dangzuo were detected by Gold Amalgam Enrichment-Atomic Fluorescence Spectrometry（GAE-AFS）.In the present study,Tibetan medicine Dangzuo was prepared by H2SO4-KNO3 digestion system and artificial gastric juice.The established method and condition of instrument were investigated.Under the optimum experimental conditions and instrumental operation parameters,the recovery（n=6）of HgS is 99.56 %（RSD=1.94%）,the limit of detection for mercury is 0.2 ng·L-1,the linear range is 0-500 ng·L-1,and r=0.999 9.Then,the total mercury and the ionic mercury in artificial gastric juice in Dangzuo samples from different Tibetan regions were assayed.The result showed that the ranges of total mercury and ionic mercury in artificial gastric juice were 3.998 0-16.735 8 mg·g-1 and 45.537 7-1 033.985 0 ng·g-1,respectively.The analytical method mentioned above is rapid and accurate for determining the amount of mercury in Tibetan medicine Dangzuo.

关 键 词：冷原子荧光 金汞齐富集 当佐 佐太 汞 人工胃液 

分 类 号：O657.3[理学—分析化学]