未经核苷酸类似物治疗的乙型肝炎病毒YMDD变异的相关研究  

The investigation of YMDD mutation of Hepatitis B virus in patients without nucleoside analogues treatment

作　　者：王怡[1] 杨丽[1] 徐菲莉[1] 

机构地区：[1]新疆医科大学附属中医医院,830000

出　　处：《内蒙古中医药》2011年第9期101-103,共3页Inner Mongolia Journal of Traditional Chinese Medicine

摘　　要：目的:研究未予核苷(酸)类似物(NA)治疗的乙型肝炎患者,其体内乙肝病毒(HBV)P基因酪氨酸-蛋氨酸-天门冬氨酸-天门冬氨酸(YMDD)变异,对于病毒自身复制及感染者肝脏生化指标的影响。方法:收集未经NA抗病毒治疗的334例HBV感染者血清,检测HBV YMDD变异,及HBV-DNA、乙型肝炎病毒E抗原(HBeAg)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)等指标。结果:体内病毒发生YMDD变异的患者,血清HBV-DNA载量,AST、ALT值均低于感染病毒未变异的患者(P<0.05)。结论:变异后的HBV病毒DNA复制能力下降,血清转氨酶低于未变异者,HBeAg阳性者发生HBV YMDD变异率高于阴性者。Objective： To explore the effect of hepatitis B virus（HBV） YMDD mutant , by comparing ALT,ASTand HBV DNA level between carders of mutant virus and tile ones with wild virus. Methords： Selected patients without nucleoside analogues treatment, to HBV YMDD mutant detection, then detected ALT, AST and HBV DNA load of all patients. Serum HBV-DNA was quantitively detected by using realtime polymerase chain reaction （RT-PCR）, ALT,AST, were detected also. Results： the patients harbored mutant virus strains had realatively lower scram HBV-DNA, ALT and AST than those harbored wild virus strains （P〈0.05） .Conclusion： the nmtant virus strain pays replication price compared with wild virus strain , therefore, the mutant virus has lower replication ability than the wild ones. the incidence rate of HBV mutant in HBeAg positive patients is higher titan in HBeAg negative ones（P〈0.05）.

关 键 词：乙型肝炎病毒 YMDD 变异 核苷酸类似物 乙肝病毒E抗原 

分 类 号：R446.1[医药卫生—诊断学]