培哚普利对自发性高血压大鼠肾脏水通道蛋白2的影响  

Effects of perindopril on kidney aquaporin-2 expression and urine aquaporin-2 excretion in spontaneously hypertensive rats

作　　者：欧阳劭[1] 陈伟[1] 匡希斌[1] 

机构地区：[1]南华大学附二医院心血管内科,湖南衡阳421001

出　　处：《中华高血压杂志》2011年第5期459-463,共5页Chinese Journal of Hypertension

摘　　要：目的探讨培哚普利对自发性高血压大鼠(SHR)肾脏水通道蛋白2(AQP2)表达和尿液AQP2排泄的影响。方法 SHR20只,随机分成2组,每组10只,分别为对照组、培哚普利治疗组,经用药后观察血压、血Na+、尿量以及尿渗透压,用放射免疫法检测血浆血管加压素(AVP)浓度,用免疫组化、RT-PCR、Western blot检测肾脏AQP2表达,用间接ELISA法检测尿液AQP2浓度。结果 SHR经培哚普利治疗8周后,血压下降(P<0·05)、血Na+水平差异无统计学意义,24h尿量增多(P<0·05)、尿渗透压低于对照组(P<0·05)。免疫组化结果显示,培哚普利治疗后肾组织AQP2表达明显减弱(P<0·05);用RT-PCR和Western blot方法检测表明,与对照组比较,治疗组肾组织AQP2 mRNA和蛋白的表达均明显减少(均P<0·05);血浆AVP浓度低于对照组(P<0·05)、尿液AQP2浓度高于对照组(P<0·05)。结论培哚普利下调SHR肾脏组织的AQP2 mRNA和蛋白表达,增加AQP2尿液排泄。Objective To investigate the effects of perindopril on renal aquaporin-2（AQP2） expression and urine aquaporin-2（U-AQP2） excretion in spontaneously hypertensive rats （SHRs）. Methods Twenty SHRs were randomized into control group（SHRctrl） and perindopril group （SHRper） （n=10 per group）. Blood and urine samples were collected from the SHR for detecting blood presssure, serum Na＋, urine volume and urine osmolality after perindopril treatment. Plasma arginine vasopressin （p-AVP） concentration was detected by radioimmunoassay （RIA）. Immuno-histochemistry, semi-quantitative real time-polymerase chain reaction （RT-PCR） and Western-blot were performed to measure the expression of kidney AQP2. U-AQP2 concentration was detected by indirect enzyme-linked immunosorbent assay （ELISA）. Results After 8 weeks, blood pressure and 24 hours urine osmolality were lower in SHRper than in SHRctrl（P0.05）, and 24-hour urine volume increased in SHRper（P0.05）, while serum Na＋ did not differ significantly between the two groups（P0.05）. Immuno-histochemistry analysis showed that the treatment resulted in marked down-regulation on renal AQP2 expression in SHRper（P0.05）. RT-PCR and Western blot also revealed significantly decreased levels of kidney AQP2 mRNA and AQP2 protein in SHRper（P0.05, respectively）. Compared with those in SHRctrl, p-AVP concentration was lower in SHRper（P0.05）, whereas U-AQP2 concentration was higher in SHRper （P0.05）. Conclusion Perindopril can reduce the expression of AQP2 in the kidney inner medullary, and accelerate the excretion of U-AQP2 in SHRs.

关 键 词：水通道蛋白2 血管加压素 自发性高血压大鼠 培哚普利 

分 类 号：R544.1[医药卫生—心血管疾病]