植物同源结构域(PHD)-finger家族转录因子OsPHD1的过量表达可提高水稻的耐逆性能  

Overexpression of a Plant Homedomain(PHD)-finger Transcription Factor,OsPHD1,Can Enhance Stress Tolerance in Rice

作　　者：刘雨[1,2] 刘春霞 李早霞 夏勉 江海洋[1] 程备久[1] 周君莉 朱苏文[1] 

机构地区：[1]安徽农业大学作物生安徽省物学重点实验室,合肥230036 [2]北京未名凯拓农业生物技术有限公司,北京100085

出　　处：《农业生物技术学报》2011年第3期462-469,共8页Journal of Agricultural Biotechnology

基　　金：国家科技支撑计划(No.2009BADA6B001);国家自然科学基金(No.11075001)共同资助

摘　　要：OsPHD1基因属于水稻(Oryzasativa)植物同源结构域(PHD)-finger转录因子基因家族。逆境(干旱、高盐和低温)处理下,水稻内源基因OsPHD1的表达量明显升高。利用农杆菌介导法将OsPHD1基因转入水稻(Oryzasativassp.japonica)中花11,获得OsPHD1的过表达植株。耐逆性实验表明,过表达OsPHD1基因使转基因株系对低温(4～8℃)、高盐(200mmol/L)和干旱胁迫(相对含水量70%～95%)的耐受性分别提高43.3%、60%和25%,且在T2中获得稳定遗传。同时转基因水稻在其他性状与对照无明显差异。在洋葱(Alliumcepa)表皮亚细胞定位实验中OsPHD1与GFP的融合蛋白定位于细胞核中,qRT-PCR结果表明,OsPHD1蛋白可能通过调节胁迫反应基因的表达来调节植物的抗逆性。研究结果提示,OsPHD1基因在水稻抗逆育种上有重要的应用前景。OsPHD1 is a member of the plant homedomain（PHD）-finger transcription factor family in rice（Oryza sative）.Expression of OsPHD1 is induced under various stresses,including drought,high salinity and cold treatments.By using Agrobacterium-mediated method,OsPHD1 was successfully introduced into rice cultivar Zhonghua 11（Oryza sativa ssp.japonica） and T1 generation of transgenic rice was achieved.The results indicated that overexpression of OsPHD1 in rice could significantly improve more than 43.3%,60 % and 25% respectively in drought（70%~95% relative water content）,high saalinity（200 mmol/L） and cold tolerance（4~8℃） of transgenic plants,and this phenotype was well confirmed in transgenic plants of T2 generation,meanwhile,transgenic plant was almost not different to control in some field characters.Subcellular localization assay of OsPHD1 by using transient expression of OsPHD1：：GFP fusion protein in onion（Allium cepa） cells revealed nuclear localization of OsPHD1 protein.Results of qRT-PCR suggested that OsPHD1 protein regulated the anti-reversibility of plants by regulating the stress response of gene expression.Results imaged that OsPHD1 gene has an important application prospect for the anti-reversibility of palnt breeding.

关 键 词：OsPHD1 转基因 非生物胁迫 亚细胞定位 

分 类 号：S511[农业科学—作物学]