抵抗素对猪前脂肪细胞增殖与分化的影响  

Effects of Resistin on Proliferation and Differentiation of Porcine Preadipocyte In vitro

作　　者：陈育峰[1,2] 刘铀[1] 陈绍红[1] 刘艳芬[1] 

机构地区：[1]广东海洋大学农学院,湛江524088 [2]广东双林生物制药有限公司实验动物中心,湛江524009

出　　处：《农业生物技术学报》2011年第3期501-506,共6页Journal of Agricultural Biotechnology

基　　金：广东海洋大学人才引进项目(No.0612053)资助

摘　　要：抵抗素对肌体脂肪代谢和能量平衡起重要调节作用。为了研究抵抗素对猪原代前脂肪细胞增殖与分化的影响,本研究从1日龄仔猪皮下脂肪组织分离前脂肪细胞,分别培养于含有0、50、100、150和200μg/L重组抵抗素的DMEM/F12培养基,根据细胞形态、甘油三酯含量及脂蛋白脂酶活性的变化检测前脂肪细胞的增殖与分化情况。结果表明,在0～100μg/L范围内,随着培养液中抵抗素浓度的增加,抵抗素能显著促进猪前脂肪细胞增殖,细胞内脂蛋白脂酶活性显著增强;当培养液中抵抗素浓度增加至100～200μg/L时,猪前脂肪细胞增殖逐渐变弱,分化作用显著增强,甘油三酯合成代谢及细胞内脂肪滴聚集增加。研究结果提示抵抗素对猪前脂肪细胞生长具有双重作用。Resistin play important roles in organism lipometabolism and energy metabolism.The present work was used to study the effects of resistin on proliferation and differentiation of porcine preadipocyte.In this paper,the preadipocytes were isolated from subcutaneus adipose tissue of 1-day-old crossbred piglet,and the preadipocytes were respectively cultivated in the DMEM/F12 culture media containing 0,50,100,150 and 200 μg/L recombinant resistin.The changes of the cell morphology were observed,and the triglyceride content and the activity of lipoprotein lipase of the cells were determined to evaluate proliferation and differentiation of porcine preadipocyte.Results showed that the proliferation of porcine preadipocytes were significantly promoted by 0 to 100 μg/L resistin supplementation in the media,the activity of lipoprotein lipase of the cells was also significantly increased.As the resistin concentration in the culture media came to 100~200 μg/L,the facilitory effect of resistin on proliferation of porcine preadipocyte was declined.Moreover,as resistin concentration increased,the differentiation of porcine preadipocyte was significantly strengthened,the intracellular triglyceride synthesize and fatty droplet aggregation were promoted simultaneously.The results indicated that the resistin has dual effects on growth of porcine preadipocyte.

关 键 词：抵抗素 前脂肪细胞 增殖 分化 

分 类 号：S831.5[农业科学—畜牧学]