外伤性癫痫病灶中caspase-10的表达  

A study on caspase-10 expression in the loci in patients with posttraumatic epilepsy(PTE)

作　　者：蔡卫林[1,2] 唐运林[3] 何鹏翔[1] 黄永华[1] 方超[1] 姜富学 陈晓霞[5] 竞花兰[1] 

机构地区：[1]中山大学中山医学院法医学系,广东广州510080 [2]中国人民武装警察部队浙江省消防总队医院,浙江杭州310011 [3]中国人民解放军第458医院,广东广州510000 [4]广州市刑事科学与技术研究所,广东广州510000 [5]温州医学院口腔系,浙江温州325000

出　　处：《中国法医学杂志》2011年第2期89-91,173,共4页Chinese Journal of Forensic Medicine

摘　　要：目的观察凋亡基因caspase-10在外伤性癫痫病灶中的表达变化,探讨其作用机制。方法收集外伤性、非外性癫痫患者手术切除的脑皮质及交通事故死亡者脑皮质各15例,运用RT-PCR技术观察比较各实验组凋亡基因caspase-10表达水平,采用荧光免疫组织化学染色技术观察各实验组凋亡基因对应蛋白的表达情况。结果外伤性癫痫组caspase-10基因和蛋白表达水平比非外伤性癫痫组和交通事故死亡组高,差异有统计学意义(P<0.05);非外伤性癫痫组caspase-10基因和蛋白表达水平亦显著高于交通事故死亡组(P<0.05)。结论凋亡基因caspase-10能促进神经元凋亡,对外伤性癫痫的发病过程有促进作用。Objective To investigate the change of apoptosis-related gene expression of caspase-10 in the patients with posttraumatic epilepsy （PTE） for its pathogenesis. Methods Samples were taken respectively from cortex in 15 patients with PTE and 15 with non-traumatic epilepsy （non-PTE） during surgical treatments. Another samples were obtained from 15 victims died from traffic accident. Caspase-10 expression was detected by RT-PCR and fluorescent immunohistochemistry. Results Caspase-10 expression was significantly up-regulated in the PTE patients as compared with those in the non-PTE patients and victims died from traffic accident （ P 〈 0. 05 ）. Similarly, caspase-10 expression was also significantly elevated in the non-PTE patients as compared with that in the victims died from traffic accident（P 〈0.05）. Conclusion Onset of PTE may be related with neuronal apoptosis by promoted caspase-10 expression.

关 键 词：法医病理学 caspase-10 RT-PCR 荧光免疫组织化学染色 外伤性癫痫 

分 类 号：D919[医药卫生—法医学]