胶体金免疫层析法检测心肌标志物在诊断急性心肌梗死中的应用  

Colloidal Gold Immunochromatography Assay for Cardiac Markers in the Diagnosis of Acute Myocardial Infarction

作　　者：刘宇[1] 周荣斌[1] 

机构地区：[1]北京军区总医院急诊科,北京市100700

出　　处：《中国全科医学》2011年第17期1903-1904,共2页Chinese General Practice

摘　　要：目的评价胶体金免疫层析法检测心肌标志物血清肌钙蛋白Ⅰ/肌酸激酶同工酶(CK-MB)/肌红蛋白诊断急性心肌梗死(AM I)的效能。方法采用双盲、自身对照的试验设计方法,分别采用科卫公司研制生产的肌钙蛋白Ⅰ/CK-MB/肌红蛋白三合一诊断试剂盒和艾康公司生产的试剂盒对140例AM I患者的血液标本进行检测。结果两种试剂检测肌钙蛋白Ⅰ、肌红蛋白和CK-MB的结果间差异无统计学意义(P>0.05)。试验试剂检测肌钙蛋白Ⅰ、肌红蛋白、CK-MB的相对敏感度分别为98.4%、99.2%和97.6%,特异度分别为94.4%、90.9%和94.1%。结论应用胶体金免疫层析法检测肌钙蛋白Ⅰ、CK-MB、肌红蛋白适于AM I的辅助诊断。Objective To evaluate the efficacy of colloidal gold immunochromatography assay for serum cardiac markers troponin I（cTnI）/creatine kinase（CK-MB）/myoglobin（Myo） in the diagnosis of acute myocardial infarction.Methods Troponin I（cTnI）,creatine kinase（CK-MB） and myoglobin of 140 confirmed patients were tested by cardiac markers produced by Kewei Company and ACON Company in a double-blind,self-controlled trial design.Results There was no significant difference between the two groups in troponin I,myoglobin and CK-MB（P 0.05）.Relative sensitivity of cTnI,myoglobin and CK-MB were 98.4%,99.2% and 97.6% respectively,and specificity were 94.4%,90.9% and 94.1% in the trial group.Conclusion Colloidal gold immunochromatography assay of cTnI,CK-MB and Myo is suitable for the diagnosis of acute myocardial infarction.

关 键 词：急性心肌梗死 胶体金免疫层析法 心肌标志物 肌钙蛋白Ⅰ 肌红蛋白 

分 类 号：R542.22[医药卫生—心血管疾病]