骨碎补总黄酮对去卵巢大鼠骨组织Cbfa1 mRNA表达的影响  

Effect of Drynaria Fortunei J.SM on Cbfa1 mRNA expression in bone of ovariectomized rats

作　　者：刘天舒[1] 李章华[1] 陈友浩[1] 柳铭[1] 金林[1] 

机构地区：[1]武汉大学人民医院骨一科,武汉430060

出　　处：《中国骨质疏松杂志》2011年第5期411-415,423,共6页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(30700854;8107146)

摘　　要：目的观察成骨细胞转录因子Cbfa1在去卵巢骨质疏松(Osteoporosis,OP)大鼠的表达,探讨骨碎补总黄酮调节骨代谢的作用机制。方法 36只Wistar雌性大鼠随机均分为假手术组、模型组和药物组,术后2、4、6、8周检测血液雌二醇(E2)水平,进行股骨和腰椎骨密度测量、组织学观察和Cbfa1mRNA表达。结果在每个时间点3组大鼠雌二醇水平均存在显著性差异(P<0.05),假手术组>模型组>对照组;比较股骨及腰椎骨密度,假手术组>模型组(P<0.05)在每一个检测的时间点,药物组在术后2周和术后8周低于假手术组(P<0.05),在术后6周和术后8周高于模型组(P<0.05),其余时间点无显著性差异。组织学检查示模型组骨组织内骨小梁减少,稀疏,间隔增大,药物组该表现较少,而假手术组骨组织内未见该现象。模型组与药物组Cbfa1 mRNA表达在术后4周均低于假手术组(P<0.05),在术后6周和术后8周,假手术组>药物组>模型组(P<0.05)。结论实验结果表明雌激素减少能导致大鼠Cbfa1表达降低,而骨碎补总黄酮能上调Cbfa1表达水平,促使骨髓间质干细胞向成骨细胞分化发挥其防治OP作用。Objective To investigate the expression of osteoblastic transfectional factor Cbfal in ovariectomized osteoporosis （OP） rats, and to explore the mechanism of regulatory effect of drynaria flavonoids on bone metabolism. Methods Thirty-six Wistar female rats were randomly divided into the sham-operated group, the model group, and the drug group. Serum estradiol （E2） level was determined on 2, 4, 6, and 8 weeks after the operation. Bone mineral densities of the femur and the lumbar vertebrae were measured. Histological observation and Cbfal mRNA expression were performed. Results Serum E2 level of the rats among the 3 groups had a significant difference on each time point, the sham-operated group 〉 drug group 〉 model group （P 〈 0. 05）. Bone mineral densities of the femur and the lumbar vertebrae in the sham-operated group were higher than those in the model group on all time points exzmined （P 〈 0.05）. Those in the drug group were lower than in the sham-operated group on 2 and 8 weeks, but higher than in the model group on 6 and 8 weeks after the operation （P 〈 0.05 ）. No significant difference was found at other time points. The histological sections showed that the trabecular bone lossed with parce and larger distance in bones of the model group. This was not obvious in the drug group, and was not seen in the sham-operated group. The expression of Cbfal mRNA in the model group and the drug group was lower than that in the sham-operated group on 4 weeks after the operation （P 〈 0.05）. The expression on 6 and 8 weeks after theoperation showed the sham-operated group 〉 drug group 〉 model group （ P 〈 0. 05 ）. Conclusion The resuhs of this study showed that estrogen deficiency resulted in reduced Cbfal gene expression in rats. Drynaria flavonoids up-regulated the level of Cbfal gene expression, and exerted OP prevention and treatment effects by stimulating osteoblast differentiation by bone marrow mesenchymal stem cells.

关 键 词：骨质疏松症 去卵巢 骨碎补总黄酮 

分 类 号：R285.5[医药卫生—中药学] R681[医药卫生—中医学]