慢性HCV感染者外周血中髓样和浆样树突状细胞频数和表型研究  

Characteristics in frequency and phenotype of myeloid and plasmacytoid dendritic cells in chronicHCV infection

作　　者：陈斯勇[1] 梁华[2] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206 [2]中国疾病预防控制中心性病艾滋病预防控制中心,北京102206

出　　处：《中华微生物学和免疫学杂志》2011年第5期448-451,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的探讨慢性HCV感染者外周血中髓样树突状细胞（mDC）和浆样树突状细胞（pDC）频数和表型的变化，并分析其与丙型肝炎临床指标间的相关性。方法采用流式细胞术检测HCV感染者及健康对照外周血中mDC和pDC的频数及细胞表面共刺激分子HLA-DR、CD83、CD86、CD40和共抑制分子PD-L1的表达水平，并分析DC频数与HCV感染者血浆病毒载量、谷丙转氨酶（ALT）的相关性。结果与健康对照组相比，HCV感染者外周血中mDC和pDC的频数明显降低（患者组分别为0．37±0．19和0．19±0．12，对照组为0．51±0．18和0．29±0．13，P〈0．05），且mDC频数与血浆HCV载量和血清AIJT水平呈负相关（r=-0．5878，P〈0．0001；r=-0．4628，P=0．003）。患者mDC和pDC表面共刺激分子HLA-DR、CD83、CD86、CD40以及共抑制分子PD-L1的表达均有不同程度升高，差别有统计学意义（共刺激分子P〈0．01，共抑制分子P〈0．05或0．01）。结论慢性HCV感染者外周血mDC和pDC频数下降，但DC表面共刺激分子和共抑制分子的表达均明显升高。该结果提示mDC数量的减少可能与HCV的慢性持续性感染有关。Objective To explore the frequencies and phenotype of myeloid and plasmacytoid dendritic cells （mDC and pDC） in chronic HCV infection and to investigate the relationships between DC frequencies and HCV viral load and serum ALT level. Methods PBMC were isolated from chronic HCV infected patients and healthy control. Multi-color flow cytometry was used to analyze the frequencies and surface marker expression on mDC and pDC. The relationship between DC frequencies and viral load and ALT level was also calculated. Results In comparison with healthy control, frequencies of mDC and pDC in chronic HCV infection were significantly decreased （0.37 ±0.19 and 0. 19±0. 12 vs 0.51±0. 18 and 0.29 ± 0.13, P 〈 0.05 ）. The frequency of mDC was negatively correlated with HCV viral load （ r = - 0.5878, P 〈 0. 0001 ） and serum ALT level （ r = - 0. 4628, P = 0. 003 ）. Both costimulatory markers （ HLA-DR, CD83, CD86, and CD40） and coinhibitory marker （ PD-L1 ） expression on mDC and pDC in HCV infection were increased （ P 〈 0.01 for costimulatory marker, P 〈 0.05 or P 〈 0.01 for eoinhibitory marker）. Conclusion The frequencies of mDC and pDC in chronic HCV infection were decreased, while the expression of costimulatory markers and coinhibitory marker were increased or not decreased in HCV infection. The decreased frequency of mDC was probably related to persistance of HCV infection.

关 键 词：丙型肝炎 髓样树突状细胞 浆样树突状细胞 流式细胞术 

分 类 号：R392[医药卫生—免疫学]