环孢A处理对脊髓损伤大鼠后肢骨骼肌RAF-1表达的影响  

Effects of Cyclosporine A on RAF-1 Expression in Skeletal Muscles of Hindlimbs in Adult Rats after Spinal Cord Transaction

作　　者：王波[1] 李玉[1] 但齐琴[2] 王廷华[2] 

机构地区：[1]昆明医学院第一附属医院神经外科,云南昆明650031 [2]昆明医学院神经科学研究所,云南昆明650031

出　　处：《昆明医学院学报》2011年第4期6-9,共4页Journal of Kunming Medical College

基　　金：国家自然科学基金资助项目(30260125)

摘　　要：目的探讨环孢霉素A处理对脊髓损伤大鼠后肢骨骼肌RAF-1 mRNA表达的影响.方法成年SD大鼠分SCT组(T9横断),和SCT后给予环孢霉素A处理组.脊髓横断组用环孢霉素A处理(1 mg/200 g)7 d后处死动物.取后肢骨骼肌用免疫组化染色(n=5)确定RAF-1 mRNA在后肢骨骼肌的定位分布.RT-PCR技术检测RAF-1 mRNA在损伤脊髓大鼠后肢骨骼肌的表达变化(n=8).结果与手术组比较(0.95±0.13),环孢霉素A处理(0.75±0.14)导致RAF-1 mRNA在损伤脊髓大鼠后肢骨骼肌的表达明显下调(P<0.05);RAF-1蛋白产物主要分布在细胞浆.结论环孢霉素A处理能有效下调脊髓损伤大鼠后肢骨骼肌RAF-1 mRNA表达水平.Objective To investigate the effects of cyclosporine A on RAF-1 expression in the skeletal muscles of hindlimbs in adult rats after spinal cord transaction.Methods Sprague-Dawley（SD）rats were randomly divided into operation group（T9 transection）and CSA treatment group.After spinal cord transection（SCT）was performed,CSA（1 mg/200 g）was intrapentoneously injected one time every day to rats in CSA treatment group till 7 days after SCT.The spinal cords were harvested,then RT-PCR（n=8）and immunohistochemistry（n=5）were respectively performed to detect the levels of RAF-1 mRNA and localization of raf protein in the skeleton muscle.Results After CSA treatment,a significant decrease in the expression levels of RAF-1 mRNA（0.75 ±0.14）was found at 7day post operation,compared with that of the SCT rats（0.95±0.13）（P〈0.05）.RAF-1 protein was distributed in the cytoplasma.Conclusion CSA can downregulate the expression of RAF-1 mRNA in skeleton muscles of hindlimbs in rats following spinal cord injury.

关 键 词：环孢霉素A 脊髓全横断 RAF-1mRNA 免疫组化 RT-PCR 大鼠 

分 类 号：R651[医药卫生—外科学]