RT-PCR和RFLP对我国部分传染性支气管炎病毒分离株分型  

Genotying of Infectious Bronchitis Virus Isolates Using Reverse Transcription-Polymerase Chain Reaction and Restriction Fragment Length Polymorphism

作　　者：尹燕博[1] 邹勇[2] 邰友华 徐兰芳[2] 吴时友[1] 蒋正军[1] 康顺文 龚振华[1] 郭福生[1] 蔡丽娟[1] 马洪超[1] 孙淑芳[1] 

机构地区：[1]农业部动物检疫所,山东青岛266032 [2]江西农业大学,江西南昌330000 [3]山东诸城市对外贸易集团公司

出　　处：《中国兽医学报》1999年第6期535-538,共4页Chinese Journal of Veterinary Science

摘　　要：用2 对引物分别对4 株传染性支气管炎病毒(IBV)标准毒株(Gray、M41、H120、MA5)和5 株国内地方分离株(KF8、18KF7、QF3、XF7、XF18)进行RT-PCR,结果均获得了与预期大小一致的片段。对此2 种PCR 产物进行了限制性片段长度多态性(RFLP)分析,分别用3 种限制性内切酶(HaeⅢ、HinfⅠ、PvuⅡ)进行酶切,根据不同的酶切图谱将测试的IBV 毒株分为不同的基因型。2 对引物的PCR 产物酶切分型结果基本一致。9 株IBV 毒株分为3 种基因型,即H120、M41、XF7、XF18、MA5 和KF8 属于Ⅰ型,Gray 属于Ⅱ型,QF3、18KF7Two pairs of oligonucleotides(primer A, primer B) were used to detect 4 reference strains (Gray, H120, M41 and MA5) and five IBV isolates(KF8, 18KF7, QF3, XF7 and XF18) by reverse transcription polymerase chain reaction(RT PCR). All strains gave the predicted products. The PCR products were digested with 3 restriction endonuclease(HaeⅢ, HinfⅠand pvuⅡ). Different IBV strains were grouped according to their restriction fragment length polymorphism(RFLP). The RFLP analysing of PCR products about B1/B2 primers agreed with the one about A1/A2 primers. Nine strains or isolates were typed into three groups. Group Ⅰ involved H120, M41, XF7, XF18, MA5 and KF8. Gray was in group Ⅱ. Isolate QF3 and 18KF7 belonged to group Ⅲ.

关 键 词：传染性支气管炎 病毒株 RT-PCR 基因型 RFLR 

分 类 号：S852.65[农业科学—基础兽医学]