细粒棘球蚴细胞系(13G-5)细胞代谢抗原的免疫原性和反应原性  

Immunogenicity and Reactionogenicity of Excretory Antigens Derived from the Cell Line of E.granulusus

作　　者：陆家海[1] 李德昌[2] 郭中敏[3] 张守竹[1] 冯德元[1] 郭固[3] 

机构地区：[1]兰州军区乌鲁木齐总医院,新疆乌鲁木齐830000 [2]解放军农牧大学动物医学系 [3]新疆畜牧科学院兽医研究所

出　　处：《中国兽医学报》1999年第6期566-568,共3页Chinese Journal of Veterinary Science

基　　金：军队医药卫生青年基金

摘　　要：应用培育成功的人源细粒棘球蚴细胞系(13G-5)细胞的天然代谢抗原,接种昆明小鼠,于接种后第14天给免疫小鼠接种人源细粒棘球蚴原头节约1 000 个,于200 d 剖检观察有无包囊形成,病理切片鉴定;并用该抗原作为诊断抗原,使用间接血凝(IHA)、琼脂扩散(AGD)试验,检测临床确诊的细粒棘球蚴病人血清。以多房棘球蚴病人血清、细颈囊尾蚴羊血清及正常人血清作为对照。结果表明,该抗原能使60% 的小鼠获得完全抵抗细粒棘球蚴原头节攻击的能力,未完全获得保护的小鼠形成包囊的数量、大小较对照组明显减少,且为不育囊;使用该抗原IHA 检测31 份血清,阳性24 份,阳性率77.42% ,AGD检测30 份血清,阳性13 份,阳性率43.33% ,2 种方法对多房棘球蚴病人血清和细颈囊尾蚴羊血清及正常人血清不起反应。Mice were injected subcutaneously with the excretory products of cell line E.granulusus as excretory antigen, and were challenged with 1000 protocoleces 2 weeks later. 200 days after infection, 60% of vaccinated mice obtained complete protection; and it was observed that the number and size of the cysts in other vaccinated mice were fewer and smaller than that in control group. When using the above products as a diagnostic antigen to detect the antibodies in sera of persons infected with E.granulusus, the sensitivity is 43.33% 77.42% by AGD and IHA respectively with the specificity of 100%.

关 键 词：细粒棘球蚴 细胞系 抗原 免疫原性 反应原性 

分 类 号：S852.732[农业科学—基础兽医学]