昆明小鼠早期胚胎发育时程  

The Chronological Events of Early Embryonic Development in Kunming Mice

作　　者：李子义[1] 谭景和[1] 孙兴参[1] 刘忠华[1] 李光鹏[1] 周琪[1] 贺桂馨[1] 

机构地区：[1]东北农业大学生物工程系,黑龙江哈尔滨150030

出　　处：《中国兽医学报》1999年第6期597-601,共5页Chinese Journal of Veterinary Science

基　　金：黑龙江省科委资助

摘　　要：以昆明种小鼠为对象,在严格控光条件下,对交配后阴道栓形成时间、排卵时间、精子入卵与原核形成时间以及2-细胞至囊胚的发育时程等进行了系统研究。结果如下:(1)从注射hCG 当天(0 d)21:00(hCG后6 h)至次日(第1 天)1:00(hCG 后10 h),有84.2% 的超排小鼠形成阴道栓;(2)大部分小鼠(66% ～100% )排卵从hCG后第1 天2:00(hCG 后11 h)开始,至3:00～4:00(hCG后12～13 h)结束;(3)从hCG 后第1 天7:00(hCG后16 h)至11:00(hCG 后20 h),小鼠卵受精率为5.6% ～82.4% ;(4)从hCG后第1 天7:00(hCG 后16 h)至11:00(hCG 后20 h),原核形成率为4.4% ～67.5% ;(5)从hCG 后第1 天21:00(hCG 后30 h)至第2 天1:00(hCG后34 h),2-细胞形成率为14.9% ～83.1% ;hCG后第2 天19:00(hCG后52 h)至23:00(hCG后56 h),4-细胞形成率为12.9% ～85.7% ;hCG 后第3 天6:00(hCG后63 h)至10:00(hCG 后67 h),8-细胞形率为12.8% ～88.2% ;(6)从hCG 后第3 天10:00(hCG 后67 h)至24:00(hCG后81 h),8～16 细胞(早期桑葚胚)形成率由100% 下降到42.9% ;从hCG 后第3 天24:00(hCG 后81 h)至第4 天4:00(hCG后85 h),17～32 细胞(晚期桑葚胚)形成率由57.1% 增加到90.0% ;(7)从hCG 后第4 天6:00(hCG后87 h)至8:00(hCG后89 h),囊胚形成率从11.4% 上升到60.9% 。Under a strict control of light/dark cycle, the chronological events, such as formation of vaginal plug,ovulation, sperm penetration, pronuclei formation and development of 2 cell to blastocyst during development of kunming mice, were systematically studied. Results were as follows: 1. The time for appearance of vaginal plug was 6 10 h after injection of hCG in 84.2% mice. 2. Ovulation time was 11 13 h after injection of hCG in 60.0% 100.0% of mice. 3. Time for sperm penetration was 16 20 h after injection of hCG in 5.6% 82.4% of mice. 4. Time of pronuclei formation was 16 20 h aftar injection of hCG in 4.4% 67.5% of mice. 5. Time of 2 cell formation was 30 34 h after injection of hCG in 14.9% 83.1% of mice. 6. Time of 4 cell formation was 52 56 h after injection of hCG in 12.9% 85.7% of mice. 7. Time of 8 cell formation was 63 67 h after injection of hCG in 12.8% 88.2%. 8.Formation time of 8 16 cell (early morula) was 67 81 h after injection of hCG and the time for 17 32 cell (late morula) was 81 85 h after hCG injection. 9. Time for blastulation was 87 89 h afater injection of hCG.

关 键 词：发育时程 早期胚胎 小鼠 胚胎发育 

分 类 号：S865.130.3[农业科学—野生动物驯养] S814.6[农业科学—畜牧兽医]