汉族人群p21codon31单核苷酸多态性与膀胱癌发生关系的研究  

Association of p21codon31 Polymorphism and Susceptibities in Bladder Cancer

作　　者：姬学义 田沛[2] 田孝华[1] 李健[1] 彭浩[1] 

机构地区：[1]河南省周口市中心医院泌尿外科,466000 [2]郑州大学医学院第一附属医院泌尿外科,450003

出　　处：《中国医疗前沿》2011年第9期1-2,共2页China Healthcare Innovation

摘　　要：目的探讨p21codon31单核苷酸多态性与膀胱癌遗传易感性之间的关系。方法应用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)方法检测90例膀胱癌患者和110例健康对照个体外周血DNA p21codon31单核苷酸多态性的分布情况。结果膀胱癌与对照组p21codon31的A(arginine)、C(serine)等位基因频率分别为73.89%、22.21%及56.82%、43.18%,两组比较差异有显著性(P<0.05);携带突变(Ser/Arg和Arg/Arg)基因型与野生(Ser/Ser)基因型两组比较,膀胱癌患病风险明显增加(OR,3.375;95%CI,1.553-7.336);膀胱癌按WHO病理分级比较,G1-G2级与G3级比较差异无显著性(P>0.05),按临床分期比较,浅表性癌与浸润性癌突变比较差异也无显著性(P>0.05);G1-G2级膀胱癌OR,2.625(95%CI,1.071-6.432);G2级OR,3.875(95%CI,1.102-13.627);浅表性癌OR,2.688(95%CI,1.038-6.961);浸润性癌OR,3.469(95%CI,1.139-10.564)。结论 p21codon31单核苷酸多态性与中原地区汉族人群膀胱癌的发病风险存在相关性,可能是膀胱癌患病的易感基因。Objective To investigate the association of p21codon31 gene polymorphism with the susceptibility to bladder cancer. Methods The single nucleotide polymorphisms（SNPS） in codon 31 of p21 gene were analyzed by polymorphism chain reaction-restriction fragment length polymorphism（PCR-RFLP） in 90 bladder cancer patients and 110 healthy controls. Results The frequency of A（arginine）, C（serine） allelotype among bladder cancer patients and healthy controls was 73.89%, 22.21% and 56.82%, 43.18%, significant difference was shown between two groups（P〈0.05）, Bladder cancer patients with Ser/Arg and Arg/Arg genotypes were at a significant 3.375-fold increased risk of bladder cancer when compared to individuals with the Ser/Ser genotype（95%CI, 1.553-7.336）, When the bladder cancer patients were grouped according to tumor grade, G,-G2 mutant compared to G3, there was no significant difference was shown between two groups（P〉0.05）. When were grouped according to tumor stage, there was no significant difference（P〉0.05）. Patients with G,-G2 and G3 mutant were at a significant 2.625-fold and 3.875-fold increased risk of bladder cancer when compared to ecotype（95%CI, 1.553-7.336）. Conclusions p21codon31 polymorphism associated with development of bladder cancer, and may be a risk factor for the susceptibility of the bladder cancer.

关 键 词：膀胱癌 P21基因 多态现象 

分 类 号：R392.1[医药卫生—免疫学] R737.14[医药卫生—基础医学]