PHBV复合HA纳米纤维材料修复兔桡骨缺损的实验研究  

Study of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/hydroxylapatite composite nanofibers for repairing rabbit radial defect

作　　者：张晓峰[1] 蒋赞利[1] 吕兰欣[2] 黄宁平[2] 陈娟 王正[1] 

机构地区：[1]东南大学附属中大医院骨科,江苏南京210009 [2]东南大学生物科学与医学工程学院生物电子学国家重点实验室,江苏南京210004 [3]武警南京医院病理科,江苏南京210028

出　　处：《东南大学学报（医学版）》2011年第3期407-413,共7页Journal of Southeast University(Medical Science Edition)

基　　金：科技部国际科技合作项目(2008DFA51180)

摘　　要：目的:探讨羟基丁酸-羟基戊酸共聚物复合羟基磷灰石(PHBV/HA)的纳米纤维材料修复骨缺损的能力。方法:将40只新西兰兔制备单侧桡骨中段10 mm的骨缺损模型,随机分为3组:实验组植入PHBV/HA的纳米纤维材料,对照组植入硫酸钙人工骨,各16只;空白对照组8只。在术后相应时间段分别进行大体观察,行放射学、组织学及扫描电镜检查,进行生物力学测试,观察其成骨能力、生物降解性、生物相容性等指标。结果:实验组术后4～8周植入材料处有片状密度增强,截骨处有骨痂向缺损区生长,骨皮质不连续,仍有骨性缺损,术后12～16周新生皮质骨与宿主皮质骨自然连接,骨缺损已完全修复。新生骨显示出正常骨干结构,髓腔再通。与对照组相比,8周之前在新生骨的形成及修复骨缺损方面的作用相差不多,差异无统计学意义(P>0.05),而到12周之后与对照组相比,实验组体现了更好的成骨能力,差异有统计学意义(P<0.05)。结论:PH-BV/HA的纳米纤维材料具有良好的生物相容性及骨传导性,是一种良好的修复骨缺损的植入材料。Objective： To investigate the capability of poly（3-hydroxybutyrate-co-3-hydroxyvalerate）/hydroxylapatite（PHBV/HA） composite nanofibers for healing bone defect.Methods：Forty New Zealand white rabbits were used to induce 10 mm radius segmental defects,then randomized into 3 groups.PHBV/HA composite nanofibers were implanted into the 16 rabbits radial defects as experiment group,calcium sulfate bone graft substitutes were implanted into the 16 rabbits radial defects as control group,nothing was implanted into the 8 rabbits radial defects as blank control group.After the operation,the bone formation capability,biodegradation,biocompatibility were evaluated through gross observation,radiology,histology,SEM and biomechanical tests at certain time point.Results：4-8 weeks after implantation,the density of the implantation region increased,osteotylus generated from both osteotomy sides to implantation region,new cortical bone was not continuous,bone defect still remained;12-16 weeks after implantation,new cortical bone connected with the host cortical bone naturally,the bone defects were perfectly healed,new bone had normal structure and the medullar cavity regenerated.The difference of healing bone defect was not significant before 8 weeks（P0.05）between PHBV/HA composite nanofibers group and calcium sulfate bone group.The capability of bone formation of PHBV/HA composite nanofibers was significantly better than that of calcium sulfate bone graft substitutes（P0.05）.Conclusion：PHBV/HA composite nanofibers have favourable biocompatibility and osteoconduction,can be used as a kind of ideal bone substitute for repairing bone defects.

关 键 词：羟基丁酸-羟基戊酸共聚物复合羟基磷灰石的纳米纤维材料 桡骨缺损 硫酸钙 

分 类 号：R318.08[医药卫生—生物医学工程] R-332[医药卫生—基础医学]