基于化学衍生化技术提高LC-MS^n在生物体液中药物定量检测灵敏度的研究进展  

Research progress of enhancing quantitative sensitivity by using LC-MS^n with derivatization method in bio-matrices

作　　者：李迎[1] 徐丽丽[1] 阮金秀[1] 张振清[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《药学学报》2011年第6期637-641,共5页Acta Pharmaceutica Sinica

基　　金："国家重大新药创制"科技重大专项综合性新药研究开发技术大平台资助项目(2009ZX09301-002);"国家重大新药创制"科技重大专项资助项目(2009ZX09103-130);单元平台资助项目(2009ZX09304-004)

摘　　要：液相色谱-质谱联用(LC-MSn)的高灵敏度和高专属性在药物研究特别是药物代谢研究中发挥着重要作用。但是随着高活性低剂量药物和特殊结构类型化合物的出现,液质联用的应用也受到限制。通过衍生化改变药物的结构,从而改变其理化性质,在液质联用检测中可以提高离子化效率,降低基质抑制和减少无机盐及内源性杂质的干扰。本文简要综述了衍生化和液质联用技术应用于生物体液中药物定量检测灵敏度提高方面的最新进展。Liquid chromatography/mass spectrometry（LC-MSn） has been essential to a large number of quantitative analytical applications in drug research,and especially in the drug PK/PD research,due to its high sensitivity and high specificity.But following the appearance of drugs with high activity and low dosage and the especial structural compounds,a number of limitations of LC-MSn have been noted.Derivatization changes the structure of drugs and therefore changes their physical and chemical properties,resulting in high ionization efficiency,low matrix effect and low disturbance by inorganic salts and endogenous compounds in LC-MSn.In this article,recent progress in the research of the chemical derivatization strategy with LC-MSn is reviewed on breakthrough of some LC-MSn limitations,in particular focusing on the applications involving some drugs in bio-matrices.

关 键 词：化学衍生化 LC-MSn 官能团 灵敏度提高 

分 类 号：R917[医药卫生—药物分析学]