葛根素对脐带间质干细胞增殖与成骨分化的作用  

Effects of puerarin on proliferation and differentiation of umbilical cord mesenchymal stem cells into osteoblasts in vitro

作　　者：蔡花[1] 吴乐乐[1] 孙晓春[1] 朱伟[1] 钱晖[1] 胡嘉波[1] 许文荣[1] 

机构地区：[1]江苏大学基础医学与医学技术学院,江苏镇江212013

出　　处：《药学学报》2011年第6期738-741,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30471968);江苏省自然科学基金(BK2008232);江苏大学博士创新基金(1293000419)

摘　　要：葛根素是从豆科植物野葛的干燥根中提取的一种异黄酮类化合物[1]。已广泛应用于临床,可治疗多种疾病,如冠心病、心绞痛、心肌梗死等[2？4]。文献报道葛根素能够促进大鼠成骨细胞增殖,抑制破骨细胞的骨吸收活性。This study is to investigate the effects of puerarin on the proliferation and differentiation of umbilical cord mesenchymal stem cells（MSCs） into osteoblasts.Umbilical cord MSCs were cultured by tissue adherence and the third passage of cells was used in the experiment.The effect of puerarin on proliferation of umbilical cord MSCs was measured with MTT.The effects of puerarin on umbilical cord MSCs were evaluated by ALP immunohistochemisty and von kossa staining.The OD value decreased with the increase of puerarin concentration.On 7th day,ALP expression of puerarin group was higher than that of control group.On 14th day,ALP staining showed that the positive rate of puerarin group was higher than that of control group.Von kossa staining showed the quantity of calcium nodules was higher in puerarin group than that of control group.Puerarin can promote the umbilical cord MSCs to differentiate into osteoblasts and has an effect on the proliferation of umbilical cord MSCs.

关 键 词：葛根素 脐带间质干细胞 成骨分化 

分 类 号：R963[医药卫生—微生物与生化药学]