葫芦种子传黄瓜绿斑驳花叶病毒的检测  

Detection of the Cucumber green mottle mosaic virus in seeds of bottle gourd

作　　者：秦碧霞[1,2] 蔡健和[1] 陆秀红[1] 林林 廖富荣[4] 刘志明[1] 

机构地区：[1]广西农业科学院植物保护研究所,南宁530007 [2]广西作物遗传改良生物技术重点开放实验室,南宁530007 [3]广西玉林市农业科学研究所,玉林537000 [4]厦门出入境检验检疫局,厦门361012

出　　处：《植物保护》2011年第3期109-112,共4页Plant Protection

基　　金：广西科学基金项目(桂科青0640024);国家质检总局科技计划项目(2005IK0061)

摘　　要：从感染黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)的葫芦植株上收取种子,通过苗期症状观察法、双抗体夹心酶联免疫吸附法(DAS-ELISA)、免疫捕获反转录PCR(IC-RT-PCR)法测定葫芦种子的带毒情况,并用生物学接种方法测定葫芦种子携带病毒的侵染活性。苗期症状观察法结果表明,199株幼苗有2株表现花叶斑驳症状,种子传毒率为1.01%;而利用DAS-ELISA和IC-RT-PCR法随机检测30粒葫芦病株种子,CGMMV检出率为100%。种子各部位携带的CGMMV接种葫芦表现典型的花叶斑驳症状,表明葫芦种子携带的CGMMV具有侵染活性。DAS-ELISA检测葫芦种子CGMMV的灵敏度为1/5120种子研磨液。The seed transmission rate of Cucumber green mottle mosaic virus （CGMMV） in the bottle gourd Lagenaria siceraria was detected based on seedling symptoms, DASELISA and immunocapture RTPCR （ICRTPCR）. Seed transmission ratio of CGMMV in bottle gourd from the viruscontaminated seeds was 1.01%, while seedborne ratio of CGMMV in bottle gourd seeds tested was 100% by DASELISA and ICRTPCR. CGMMV in bottle gourd seeds was demonstrated to have infection activity by biological inoculation. The sensitivity of DASELISA used for detection of CGMMV in the seed sap of bottle gourd was 1/5120.

关 键 词：黄瓜绿斑驳花叶病毒 种子带毒 检测 

分 类 号：S432.41[农业科学—植物病理学]