PGPR芽孢杆菌B-1菌株的鉴定及其应用效果  

Applied Effects and the Identification of PGPR Bacillus B-1 Strain

作　　者：张爱民[1] 张双凤[1] 赵钢勇[1] 张瑞英[2] 

机构地区：[1]河北大学生物技术研究中心,河北保定071002 [2]河北大学生命科学学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2011年第3期294-298,共5页Journal of Hebei University(Natural Science Edition)

基　　金：河北省科技支撑项目(062071250-2)

摘　　要：从国外产品中分离得到一株PGPR菌株,编号为B-1;观察了该菌株的形态大小和菌落形态,进行了生理生化反应,通过热变性法测定其G+C(鸟嘌呤-胞嘧啶)摩尔分数为47.2%,鉴定该菌株为短芽孢杆菌(Bacillus brevis);通过小麦水培实验研究了该菌株对小麦株高和鲜重的影响,地上部鲜重较对照增加10.64%,株高较对照增加6.75%;玉米盆栽实验证实该菌株实验效果优于胶质芽孢杆菌菌株,株高较对照增加8.0%,叶面积增加5.6%.A PGPR strain named B-1 was separated from foreign products, the morphology of the cell and the clony was observed and physiological and biology-chemical reaction were made. DNA G＋C tool% was 47.2% by the method of thermal denaturation, so the strain was identified as Bacillus brevis; The height and the fresh weight was studied by the experiments of wheat inoculated with it. The fresh weight of wheat was increased by 10.64% and the plant height was increased by 6.75%. The maize pot experiment results showed that significant increasing effect could be got, plant height and leaf area could be increased than that of the control and Bacillus mucilaginosus. Maize plant height could be increased by 8.0% and the leaf area could be increased by 5.6 %.

关 键 词：筛选分离 短芽孢杆菌 小麦水培实验 玉米盆栽实验 

分 类 号：Q93-331[生物学—微生物学]