HBx蛋白对α干扰素诱导的MxA蛋白表达的影响  

Effects of Hepatitis B virus X protein on the expression of interferon-induced MxA protein

作　　者：管世鹤[1] 潘颖[1] 杨凯[1] 沈继龙[2] 杨东亮[3] 

机构地区：[1]安徽医科大学第二附属医院检验科,湖北武汉430030 [2]安徽医科大学人兽共患病安徽省重点实验室,安徽合肥230032 [3]华中科技大学同济医学院附属同济医院临床免疫研究室,湖北武汉430030

出　　处：《中国药理学通报》2011年第6期797-800,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30600522);安徽省卫生厅科学基金资助项目(No2010C057)

摘　　要：目的探讨乙型肝炎病毒(HBV)HBx蛋白(HepatitisB virus X protein)对α干扰素(IFN-α)诱导的抗病毒蛋白的影响及相关机制。方法以表达HBx蛋白的重组质粒FL1-145HBx转染人肝胚瘤细胞株HepG2细胞,经IFN-α处理后,RT-PCR法分析细胞内抗病毒蛋白MxA和JAK-STAT信号转导途径分子STAT1 mRNA表达水平,同时运用免疫印迹检测细胞内HBx、p-ERK、p-STAT1和t-STAT1等蛋白的表达。结果转染细胞内MxA、STAT1的mRNA和p-STAT1、t-STAT1的蛋白表达水平明显减少(P<0.05);而ERK抑制剂PD98059预处理后,转染细胞内MxA、STAT1 mRNA水平能够恢复至转染前的表达水平。结论 HBx蛋白很可能通过影响IFN-αJAK-STAT信号转导途径分子而抑制抗病毒蛋白MxA的表达;ERK信号转导途径的活化可能参与这一抑制过程。Aim To explore the effects of Hepatitis B virus X（HBx） protein on the antiviral proteins induced by Interferon-α and its possible mechanisms.Methods HepG2 cells were transfected with FL1-145HBx plasmid,and the mRNA levels of MxA,STAT1 were assessed by RT-PCR after treated with IFN-α.Meanwhile,the expression of HBx,p-ERK,p-STAT1 and t-STAT1 proteins were detected by Western blot.Results The expression levels of total STAT1,phosphorylated STAT1 and STAT1 mRNA were down-significantly in transfected cells（P0.05）.However,these proteins were restored to the expression levels of control groups when the transfected cells were pretreated by ERK inhibitor PD98059.Conclusion HBx protein probably influences the expression of IFN-α JAK-STAT signal pathway molecules and antiviral protein MxA.Moreover,the activation of ERK signal transduction pathway may be involved in the procedure.

关 键 词：肝炎病毒 乙型 HBX蛋白 干扰素 MXA蛋白 信号转导途径 细胞株 

分 类 号：R341.1[医药卫生—基础医学] R373.21