人类胚胎干细胞分化研究  

Differentiation of human embryonic stem cell

作　　者：刘超[1] 刘丽华[2] 方圣云[3] 

机构地区：[1]安徽医科大学基础医学院组胚教研室,安徽合肥230032 [2]安徽医科大学临床药理研究所,安徽合肥230032 [3]马里兰大学生物医学工程和技术中心

出　　处：《中国药理学通报》2011年第6期831-834,共4页Chinese Pharmacological Bulletin

基　　金：美国马里兰州干细胞研究基金(No2007-MSCRFE-0139-01)

摘　　要：目的分别观察神经分化培养液和视黄酸对人类胚胎干细胞分化的作用。方法采用无滋养层细胞方法培养H9细胞,用化学成分确定的神经分化培养液诱导H9细胞神经分化,检测神经细胞特征蛋白表达;用视黄酸(RA)培养液诱导H9细胞分化,检测分化过程中内质网应激蛋白BIP蛋白表达变化。结果神经分化培养液诱导H9细胞分化成神经细胞;RA诱导的H9细胞分化过程中,BIP的表达随分化上调。结论人类胚胎干细胞分化提供了衍生神经细胞的潜在细胞来源和发育研究的平台。Aim To study the effect of neural induction medium and RA on human embryonic stem cell differentiation.Methods H9 cells maintained on Matrigel in mTeSRTM1 medium were converted to neural cells with chemically defined neural induction medium to test the expression of neural marker proteins,and were treated with retinoid acid（RA） to test expression of BIP,an endoplasmic reticulum stress related protein.Results Neural cells were generated from H9 cells successfully with neural induction medium,and expression of BIP was upregulated during the H9 cell differentiation induced by RA.Conclusions Appropriate HESC differentiation provides the potential cell source for generating neural cells and a platform for developmental study.

关 键 词：人类胚胎干细胞 H9细胞 神经分化 视黄酸 BIP 发育 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学] R329.2[医药卫生—基础医学]