人体退变椎间盘中PDGF-A、PDGFR-α的表达及生物学意义  

Expression and biological significance of PDGF-A and PDGFR-α in human degenerated intervertebral discs

作　　者：谢洋[1] 刘银华[2] 周涛[1] 陈小强[1] 何仿[1] 

机构地区：[1]马鞍山市人民医院骨科,安徽马鞍山243000 [2]皖南医学院弋矶山医院病理科,安徽芜湖241000

出　　处：《颈腰痛杂志》2011年第3期177-180,共4页The Journal of Cervicodynia and Lumbodynia

基　　金：马鞍山市卫生局科教基金(编号08007)

摘　　要：目的观察人体退变椎间盘组织中血小板源性生长因子-A(PDGF-A)、血小板源性生长因子受体-α(PDGFR-α)的表达,探讨其生物学意义。方法免疫组化(S-P)法分别检测PDGF-A、PDGFR-α、增殖细胞核抗原(PCNA)在正常对照组(A组,n=17)、退变椎间盘组(B组,n=51)中的表达;TUNEL法检测两组椎间盘细胞凋亡情况。结果 PDGF-A、PDGFR-α在B组中表达低于A组,两组之间差异有统计学意义(P<0.05);细胞增殖指数(PI)在B组数值低于A组,细胞凋亡指数(AI)在B组数值高于A组,两组之间差异有统计学意义(P<0.05);PDGF-A、PDGFR-α的表达与PI正相关、与AI负相关(P<0.05);PI、AI值在PDGF-A、PDGFR-α阴性和阳性组中差异有统计学意义(P<0.05)。结论 PDGF-A、PDGFR-α的异常表达与人体椎间盘退变密切相关;PDGF-A、PDGFR-α促进细胞增殖,抑制细胞凋亡,在人体椎间盘退变过程中起了重要的作用,其异常表达可能导致椎间盘退变的发生。Objective To observe the expression of PDGF-A and PDGFR-α in human degenerated intervertebral discs and investigate its biological significance.Methods 68 patients were divided into two groups：17 cases of normal intervertebral discs（group A）,51 cases of degenerated intervertebral discs（group B）.The expression of PDGF-A、PDGFR-α,PCNA and apoptosis were detected by immunohistochemical and TUNEL.Results The expression rate of PDGF-A and PDGFR-α in group B was lower than group A,the difference was significant between them（P0.05）.PI in group B was significant lower than that in group A（P0.05）.AI in group B was significant higher than that in group A（P0.05）.The expression of PDGF-A and PDGFR-α had positive correlation with PI and negative correlation with AI（P0.05）.There was significant difference in PI and AI between positive expression group and negative expression group（P0.05）.Conclusion Abnormal expression of PDGF-A、PDGFR-α is closely related to degenerative intervertebral discs;PDGF-A and PDGFR-α promote cell proliferation and inhibit intervertebral discs cell apoptosis,which has an important effect on the degeneration of intervertebral discs.Abnormal expression of PDGF-A、PDGFR-α can induce degeneration of intervertebral discs.

关 键 词：血小板源性生长因子-A 血小板源性生长因子受体-α 椎间盘退变 

分 类 号：R681.53[医药卫生—骨科学]