伏隔核DBS对大鼠海洛因强化作用的影响  

Effects of deep brain stimulation of nucleus accumbens on heroin reinforcement in rats

作　　者：王冉[1] 周洪语[1] 郭烈美[1] 周建鹏[1] 徐纪文[1] 王桂松[1] 周文华[2] 刘昱[3] 张富强[2] 刘惠芬[2] 江基尧[1] 

机构地区：[1]上海交通大学医学院附属仁济医院神经外科 [2]宁波市戒毒研究中心暨宁波大学医学院行为神经科学研究中心 [3]宁波大学医学院

出　　处：《立体定向和功能性神经外科杂志》2011年第1期30-35,共6页Chinese Journal of Stereotactic and Functional Neurosurgery

基　　金：国家自然科学基金面上项目(编号:30600634)

摘　　要：目的观察脑深部电刺激伏隔核核心部对大鼠海洛因强化作用的影响。方法用固定比率程序建立大鼠海洛因自身给药模型,随机分为刺激组(6只)和假刺激组(6只),训练累进比率程序达稳定状态后,两组大鼠行双侧伏隔核核心部微电极植入。刺激组大鼠每日给予高频电刺激1h(频率130Hz,电流150μA,波宽100μs),连续10d。刺激结束后两组大鼠进行累进比率程序测试。两组大鼠在刺激前、后分别测试自发活动;累进比率程序测试结束后进行Morris水迷宫实验。结果在累进比率程序测试中,刺激组大鼠的有效鼻触数(211.17±98.31)和注射次数(10.83±1.72)均明显少于假刺激组的有效鼻触数(356.17±66.25)及注射次数(13.50±1.05),且具有统计学差异(P<0.05);两组大鼠在总活动度和水迷宫中的表现均无统计学差异(P>0.05)。结论脑深部电刺激大鼠伏隔核核心部可以明显减低海洛因的强化作用,降低大鼠获取海洛因的动机,且不会影响其活动度及学习记忆能力。Objective To investigate the effects of deep brain stimulation of nucleus accumbens on heroin reinforcement. Methods The rats were trained for heroin self--administration using fixed ratio and then progressive ratio program. Bilateral concentric bipolar electrodes were implanted into NAc when reaching the conditioning criterion. The rats were randomly divided into two groups, the stimulated group and the sham--stimulated group： the former were given stimulation for lh daily for 10 consecutive days （parameters： frequency, 130Hz; pulse duration, 100us; intensity 100uA）, the latter were prepared similarly but without stimulation. One day after DBS, the rats were subjected to a progressive ratio test. On the next day, the rats were subjected to a Morris water maze test. The locomotor activity were tested before and after DBS respectively. Results In the PR test, stimulated rats showed significantly fewer active nose-poke responses （211.17±98. 31） than sham--stimulated rats （356. 17±66.25）, this was also reflected in the number of rewards （stimulated 10. 83± 1.72 vs sham--stimulated 13. 50± 1.05, P〈 0.05）. No significant difference was found between the two groups in Morris water maze performance and locomotor activity （P〉0. 05）. Conclusion DBS of NAc significantly reduce the reinforcing effects of heroin as well as the desire for heroin, without long-term influence on motor activity, learning and memory.

关 键 词：脑深部电刺激 伏隔核 累进比率 自身给药 海洛因强化 

分 类 号：R996[医药卫生—毒理学]