骨髓间充质干细胞对INS-1细胞凋亡的影响  

Bone marrow mesenchymal stem cells protect INS-1 cells from hydrogen peroxide-induced apoptosis

作　　者：孙慧环[1] 刘福强[1] 龚蕾[1] 肖芳[1] 侯新国[1] 孙宇[1] 宋君[1] 陈丽[1] 王旭平[2] 

机构地区：[1]山东大学齐鲁医院内分泌科,济南250012 [2]山东大学齐鲁医院教育部和卫生部心血管重构与功能研究重点实验室,济南250012

出　　处：《山东大学学报（医学版）》2011年第5期6-9,14,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Z2008C05);山东省国际科技合作项目(2010GHZ20201);山东大学自主创新基金(16010159611068);山东省医药卫生科技发展计划项目(2009QW008)

摘　　要：目的探讨骨髓间充质干细胞(BMSCs)对过氧化氢(H2O2)诱导胰岛β细胞凋亡的影响。方法以H2O2作用于大鼠胰岛瘤细胞INS-1细胞构建凋亡模型,通过Transwell装置实现INS-1细胞与BMSCs共培养。实验分为4组:单独INS-1细胞培养组(A组);INS-1+H2O2处理组(B组);BMSC+INS-1Transwell共培养组(C组);BMSC+INS-1+H2O2Transwell共培养组(D组)。MTT法检测细胞存活率,Annexin-V/PI染色流式细胞技术检测细胞凋亡水平。结果 H2O2显著降低INS-1细胞存活率,引起INS-1细胞凋亡,且呈剂量依赖性,通过Tran-swell与BMSCs共培养后,由H2O2诱导的INS-1凋亡细胞比例下降,与单独H2O2刺激组相比,细胞凋亡率下降了约26.3%,差异有统计学意义(P<0.05)。结论 BMSCs通过Transwell共培养显著抑制H2O2诱导的INS-1细胞凋亡,其作用机制可能与BMSCs旁分泌作用有关,为进一步利用BMSCs防治糖尿病提供了理论依据。Objective To investigate the effect of bone marrow mesenchymal stem cells（BMSCs） on hydrogen peroxide（H2O2）-induced apoptosis in INS-1 cells.Methods The apoptotic INS-1 cells,induced by H2O2,were co-cultured with BMSCs.The experiment consisted of 4 groups： group A（control group）,INS-1 cells only;group B,treated with H2O2;group C,co-culture of INS-1 cells and BMSCs;and group D,co-culture with H2O2 treatment.Cell viability was measured by MTT.Annexin-V/PI doubled staining of flow cytometry was used to quantify the apoptosis ratio.Results INS-1 cell viability was reduced by H2O2 in a dose-dependent manner.With Transwell co-culture,H2O2 induced INS-1 cell apoptosis ratio was significantly lower than that of H2O2 group（P〈0.05）.Conclusion BMSCs can protect INS-1 cells from apoptosis induced by H2O2 and may have therapeutic potentials to prevent and treat diabetes.

关 键 词：胰岛Β细胞 凋亡 骨髓间充质干细胞 共培养 

分 类 号：R587.1[医药卫生—内分泌]