柴松遗传多样性的RAPD分析  

Analysis of Genetic Diversity of Pinus tabulaeformis f.Shekannesis by RAPD

作　　者：解庆[1] 刘志红[1] 李周岐[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北林学院学报》2011年第4期112-116,122,共6页Journal of Northwest Forestry University

基　　金：国家自然科学基金(30972382)

摘　　要：以柴松现存2个居群各50个个体为样本,利用随机扩增多态DNA(RAPD)标记研究了其遗传多样性。50条引物共扩增出349个位点,其中多态位点为321个。用POPGEN32版软件对数据进行了分析,结果表明:(1)柴松的遗传多样性水平很高。在物种水平上,多态位点百分率(PPB)为92.51%,Nei's基因多样度指数(H)为0.317 3,Shannon多样性信息指数(I)为0.478 2,总基因多样度(Ht)为0.317 4。在居群水平上,平均多态位点百分率为89.92%,H和I平均值分别为0.302 5和0.456 6;(2)居群间的遗传分化较低。居群间遗传分化系数(Gst)为0.046 7,基因流(Nm)为10.201 8,Shannon's居群分化系数((Isp-Ipop)/Isp)为0.045 2。表明柴松的遗传变异主要存在于居群内,占总变异的95%以上,居群间的遗传变异不足5%。并根据柴松的遗传多样性现状提出了相应的保护策略和措施。Genetic diversity of two extant populations of Pinus tabulaeformis f.Shekannesis were investigated by using RAPD markers.A total of 349 RAPD loci were generated from 100 individuals of 2 populations with 50 primes,of which polymorphic loci were 321.POPGENE 32 was used to analyze the data.The results showed that P.tabulaeformis f.Shekannesis had an extraordinarily high level of genetic diversity.On the species level,percentage of polymorphic loci（PPB） was 92.51%,total gene diversity（Ht）was 0.317 4,Nei＇s gene diversity（H） and Shannon＇s Information index（I） were 0.317 3 and 0.478 2 respectively.On the population level,mean percentage of polymorphic loci was 89.92%,the means of H and I were 0.302 5 and 0.456 6 respectively.A low level of genetic differentiation between populations was detected based on gene flow（Nm =10.201 8）,Nei＇s genetic diversity analysis（4.67%） and Shannon＇s diversity index（4.52%）.Finally,some management strategies of protecting this species were proposed.

关 键 词：柴松 遗传多样性 RAPD 

分 类 号：S791.254[农业科学—林木遗传育种]