LXRα激活剂T0901317对急性肺损伤大鼠肺组织MPO、TNF-α表达的影响  

Influence of liver X receptor-α activator T0901317 on expression of MPO and TNF-α in rat lung with acute lung injury

作　　者：徐静[1] 李运成[1] 何春琳[2] 肖波[1] 王建春[1] 

机构地区：[1]第三军医大学新桥医院全军呼吸内科研究所,重庆400037 [2]汉中市人民医院,陕西汉中723000

出　　处：《四川医学》2011年第5期621-623,共3页Sichuan Medical Journal

基　　金：国家自然科学基金资助项目(编号:30570808)

摘　　要：目的观察LXRα(liver X receptorα)激活剂T0901317对急性肺损伤大鼠肺组织中髓过氧化物酶(MPO)、肿瘤坏死因子-α(TNF-α)表达的影响。方法 72只雄性Wistar大鼠,按随机数字表法分为对照组、脂多糖致伤组、T0901317干预组,在1h、2h、4h、8h时相点采集大鼠肺组织测定肺组织中MPO活性、TNF-α含量,并观察肺组织病理学变化。结果与对照组比较,脂多糖致伤组各时间点MPO活性均显著升高(P<0.05),组织病理显示肺组织受损;肺组织匀浆和动脉血清中TNF-α亦显著升高。T0901317干预组MPO活性及TNF-α表达下降,肺部炎症明显减轻。结论 T0901317能够显著降低急性肺损伤大鼠肺组织MPO活性、TNF-α水平,对急性肺损伤大鼠具有保护作用。Objective To investigate inhibitory action of T0901317,a liver X receptor-α activator,on expression of MPO and TNF-α in rats with acute lung injury（ALI）.Methods 72 male Wistar rats were randomly divided into three group： control group（C group）,LPS group（L group）,T0901317 group（LT group）.The rats were killed at 1h,2h,4h,8h after treatment.The lung tissue was collected,myeloperoxidase activity,lung histopathologic changes and expression of TNF-α were observed.Results In rats with ALI,myeloperoxidase activity rised compared with control group（P0.05）,and lung injury is more severe than that in rats of control group.TNF-α increased markedly in lung homogenate and blood serum at the same period（P0.05）.However,The expression of TNF-α and myeloperoxidase activity in rat lung tissues in LT group were significantly declined.Conclusion T0901317 has played an anti-inflammatory effect through suppressing the expression of TNF-α and myeloperoxidase activity in rat lung with acute lung injury.

关 键 词：T0901317 急性肺损伤(ALI) 髓过氧化物酶(MPO) 肿瘤坏死因子-α(TNF-α) 

分 类 号：R563.8[医药卫生—呼吸系统]