荞麦子叶期幼叶过氧化物酶同工酶研究  

Study on Peroxidase Isozyme of Young Leaves of Buckwheat at Seed-leaf Stage

作　　者：张以忠[1] 邓琳琼[2] 游萍[1] 蹇黎[1] 胥献宇[1] 马检[1] 

机构地区：[1]毕节学院环境与生命科学系,贵州毕节551700 [2]毕节学院岩溶山区生态建设研究所,贵州毕节551700

出　　处：《贵州科学》2011年第3期35-39,共5页Guizhou Science

基　　金：贵州省毕节地区科技计划项目(毕科合字(2007)12号);贵州省教育厅自然科学研究项目(黔教科2010074)号;毕节学院自然科学基金重点项目(院科合字20072007号)资助

摘　　要：用聚丙烯酰胺凝胶电泳技术对荞麦属(Fagopyrum Mill)的甜荞(F. esculentum)和苦荞(F. tataricum)共19个不同收集系子叶期幼叶的过氧化物酶同工酶进行了研究。结果发现:过氧化物酶同工酶酶带共10条,其中甜荞有5条带,而苦荞为6条。酶带及聚类分析表明:甜荞和苦荞各收集系内的过氧化物酶同工酶酶带数无变化,但在酶带的深浅和宽窄等方面存在一定的差异,暗示甜荞和苦荞不同收集系间遗传变异较小。The peroxidase isozyme of 19 collections of F. esculentum and F. tataricum of genus Fagopyrum were studied by means of polyacrylamide gel eletrophoresis （PAGE）. The results showed a total of ten bands of peroxidase isozyme. Among them F. esculentum has a total of five bands and F. tataricum six bands. Enzyme bands and cluster analysis showed that the band number of peroxidase isozymes of F. esculentum and F. tataricum within the respective accessions does not change, but the darkness and breadth of the enzyme bands are different within the different accessions, suggesting that there are smaller genetic variations in different collections between F. esculenurn and F. tataricum.

关 键 词：甜荞 苦荞 过氧化物酶同工酶 凝胶电泳 酶谱 

分 类 号：Q946.5[生物学—植物学]