细胞编码microRNA在轮状病毒复制中的作用及其表达谱分析  

Profile analysis of cellular microRNAs and its role in rotavirus replication

作　　者：张记[1] 彭杨[1] 何海洋[1] 申子刚[1] 吴玉章[1] 李晋涛[1] 

机构地区：[1]第三军医大学基础部全军免疫学研究所,重庆400038

出　　处：《免疫学杂志》2011年第6期461-465,共5页Immunological Journal

基　　金：国家自然科学基金(面上项目30972716);重庆市科技攻关计划项目

摘　　要：目的研究细胞编码miRNA在轮状病毒复制中的作用,并分析轮状病毒感染对于细胞编码miRNA种类及表达的影响,结合预测初步寻找具有重要功能的单个miRNA。方法首先,利用RNAi技术成功干扰MA104细胞中miRNA生成必须的Dicer酶,利用FFA法比较Dicer干扰组与阴性对照组感染后子病毒的滴度。然后,利用miRNA微芯片技术分析MA104细胞在2株轮状病毒SA11与Wa株感染后miRNA表达变化,通过聚类分析研究变化规律。结果干扰组MA104细胞的子病毒滴度显著增高。MA104细胞编码miRNA在2株轮状病毒感染后,约有200个miRNA有不同程度的变化。结合计算机预测,初步锁定miR-512-5p,miR-490,let-7c等10个miRNA可能在轮状病毒复制中具有功能。结论轮状病毒调节其感染细胞编码的miRNA的表达谱,反过来,细胞的Dicer及细胞编码miRNA影响轮状病毒复制。There is a growing amount of evidence that miRNAs play critical roles in intricate host-pathogen interaction networks,but the involvement of miRNAs during rotavirus infection is unknown.We asked whether cellular miRNAs are important for rotavirus replication and whether rotavirus infection leads to changes in the expression of cellular miRNAs.Our result showed that when Dicer expression in MA104 cell was downregulated by siRNA,rotavirus production increased in a modest level after infection.The result of microarray showed that levels of most miRNAs did not change markedly during SA11 and Wa infection,but nearly 200 miRNAs were positively or negatively regulated.Furthermore,about 100 miRNAs were predicted targeting SA11 genome.Combined microarray and prediction result,we identified 10 miRNAs,such as miR-512-5p,miR-490,let-7c,may as candidate individual crucial miRNA in rotavirus replication.Thus,our findings suggest that Dicer and cellular miRNAs are involved in rotavirus infection and support the notion that cellular miRNAs play an important role in defending against virus infection.

关 键 词：轮状病毒 微小RNA DICER酶 RNA干扰 

分 类 号：R373[医药卫生—病原生物学]