人脐带血来源树突状细胞、巨噬细胞和单核细胞体外培养及鉴定  

In vitro culture and identification of dendritic cells,macrophages and monocytes from human cord blood

作　　者：陈琛[1] 江振友[1] 高永鹏[1] 于斌[2] 陈孝银[2] 

机构地区：[1]暨南大学医学院微免系,广州510632 [2]暨南大学医学院中医系,广州510632

出　　处：《免疫学杂志》2011年第6期491-493,498,共4页Immunological Journal

基　　金：国家自然科学基金(30973693);教育部博士点基金(20104401110003)

摘　　要：目的建立从人脐带血体外诱导扩增树突状细胞、巨噬细胞和单核细胞的方法。方法从正常人脐带血中分离出单核细胞,经3种细胞因子—重组人粒细胞-巨噬细胞集落刺激因子(rh GM-CSF)、重组人白细胞介素4(rh IL-4)和重组人肿瘤坏死因子(rh TNF-α)联合诱导培养,7 d后收获细胞,并利用光学显微镜、免疫组化的方法进行鉴定。结果培养收获了高纯度的树突状细胞、巨噬细胞、单核细胞,细胞高水平表达CD1a、CD86、CD68和CD14。结论人脐带血单核细胞经细胞因子诱导培养可以得到大量的成熟树突状细胞、巨噬细胞和单核细胞。In this study,we aimed at establishing a method to obtain dendritic cells,macrophages,and monocytes from human cord blood in vitro.The three immune cells which isolated from human cord blood,then induced by three cytokines-Recombinant human granulocyte-macrophage colony stimulating factor（rh GM-CSF）,recombinant human interleukin-4（rhIL-4）,and recombinant human tumor necrosis factor（rhTNF-α）.Seven days after,these cells were harvested and identified the cells by optical microscopy and immunohistochemical methods.The results showed that a large number of high purity dendritic cell,macrophages and monocytes were generated,which expressed high level of CD1a and CD86,CD68 and CD14.The study lays a foundation for research on in vitro experiments of mature dendritic cells,macrophages and monocytes.

关 键 词：树突状细胞 巨噬细胞 单核细胞 脐带血 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]