瑞香狼毒杀螨活性物质的分离与鉴定  

Isolation and Identification of the Principal Acaricidal Components from Stellera chamaejasme

作　　者：梁为[1] 白雪娜[2] 成军[2] 师光禄[2] 王有年[2] 王战全 

机构地区：[1]北京林业大学林学院,北京100083 [2]农业部都市农业北方重点开放实验室,北京农学院,北京102206 [3]平谷区马昌营镇人民政府,北京101200

出　　处：《园艺学报》2011年第5期947-954,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30872029);北京市自然科学基金重点项目(6071001);北京市自然科学基金项目(6092007);北京市教委重点项目(KZ201010020016);北京市教委平台建设项目;北京市属高校人才强教深化计划项目(PHR20090516,PHR200906134)

摘　　要：采用活性跟踪法,对瑞香狼毒(Stellera chamaejasme L.)中的杀螨活性成分进行研究。从瑞香狼毒乙酸乙酯提取物中分离出对朱砂叶螨(Tetranychus cinnabarinus)具有较强触杀和内吸活性的化合物Ⅳ,通过核磁共振(1H NMR,13C NMR)、红外色谱(IR)等方法对化合物Ⅳ进行鉴定,确定该化合物为东莨菪内酯(scopoletin)。杀螨活性的测定结果表明,东莨菪内酯可能是瑞香狼毒中重要的杀螨活性物质。东莨菪内酯对朱砂叶螨触杀活性的半致死浓度(LC50)值为1.267mg·mL-1,内吸活性的LC50值为7.388mg·mL-1。Using the acaricidal activity to guide the isolation,the compound Ⅳ,which had the highest contact and systemic toxicity to Tetranychus cinnabarinus,was obtained from ethyl acetate extracts of roots of Stellera chamaejasme L. The structure of compound Ⅳ was established as scopoletin by analysis of NMR（1H NMR,13C NMR）and IR data. The results of acaricidal activity analysis showed that scopoletin may be the principal acaricidal substance in the ethyl acetate extracts of S. chamaejasme extracts. And the mean lethal concentrations（LC50）of scopoletin for contact and systemic toxicity were 1.267 and 7.388 mg · mL-1,respectively. Subsequently,compound Ⅳ was analyzed by NMR and IR methods,and its contact and systemic toxicity to mites were determined. In accordance with the spectral data（1H NMR,13C NMR and IR）of compound Ⅳ,the chemical compound was identified as scopoletin. Scopoletin may be the principal acaricidal substance in S. chamaejasme extracts. And the mean lethal concentrations（LC50）of scopoletin for contact and systemic toxicity were 1.267 and 7.388 mg · mL-1,respectively.

关 键 词：瑞香狼毒 杀螨活性 朱砂叶螨 东莨菪内酯 

分 类 号：S482[农业科学—农药学] S567[农业科学—植物保护]