DSG4基因的SNPs筛查及其与裘用绵羊羊毛性状关系的研究  

Polymorphism of DSG4 Gene and Its Association with Wool Traits in Fur Sheep

作　　者：张戈[1,2] 张元跃[2] 韩建林[1,3] 关伟军[1] 李颖康 许斌 张秀陶 马月辉[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室,北京100193 [2]湖南农业大学动物科技学院,长沙410128 [3]国际家畜研究所,肯尼亚内罗毕001003 [4]宁夏农业科学院畜牧兽医研究所,银川750002

出　　处：《畜牧兽医学报》2011年第5期641-649,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：现代农业产业技术体系建设专项资金(nycytx-40-5);科技支撑计划(2006BAD13B08;2008BADB2B01);转基因生物新品种培育科技重大专项(2008ZX08009-003)

摘　　要：应用PCR-SSCP方法检测118只滩羊和82只小尾寒羊与滩羊杂交羊DSG4基因的多态性,用最小二乘法分析该基因多态性与毛股长度和弯曲数的关系。结果发现16个SNPs和1个TTG插入/缺失,其中在5′调控区和3′调控区各有1个SNP;11个SNPs位于外显子区域,其中4个为错义突变,TTG插入/缺失使丝氨酸替换为异亮氨酸并引起甘氨酸的插入/缺失;其余3个SNPs位于内含子区域。DSG4基因编码区的6处碱基变异与3′调控区的1个SNP处于完全连锁不平衡,组合基因型命名为PPRR、PQRS和QQSS。杂交羊QQSS基因型的初生肩部毛股长度、初生肩部毛股弯曲数以及二毛(1月龄左右)肩部毛股弯曲数的最小二乘均值极显著大于PPRR和PQRS基因型所对应的值(P<0.01);QQSS基因型的二毛股部毛股弯曲数的最小二乘均值显著大于PPRR基因型(P<0.05),但与PQRS基因型差异不显著(P>0.05)。滩羊QQSS基因型上述性状的最小二乘均值也大于PPRR和PQRS基因型,但差异不显著(P>0.05)。本研究初步表明,QQSS基因型可能是裘用型绵羊毛股长度和弯曲数的有利基因型,本研究的结果为阐明DSG4基因对绵羊羊毛性状的影响提供了较丰富的分子素材。By SSCP assay,polymorphisms of DSG4 gene were screened in Tan sheep（n=118） and their crossbred with Small-tailed Han sheep（n=82） in this study.The relationship between polymorphisms of DSG4 gene and length and crimp number of scapular wool plait was analyzed by least square linear model.The results showed 16 SNPs and a TTG indel in sheep DSG4 gene,in which one each SNP located at 5′UTR and 3′UTR,respectively,11 SNPs on coding region with four of them leading to amino acid substitutions,and three SNPs on introns.The TTG indel led to an amino acid substitution from Ser to Iso and also a Gly indel.Interestingly,six variations in coding region and the SNP at 3′UTR were in complete linkage disequilibrium,forming combinated genotypes named as PPRR,PQRS and QQSS.In the crossbreds,individuals with QQSS genotype had a significantly higher least square mean than individuals with genotype PPRR or PQRS on length of scapular wool plaits at birth（P0.01）,crimp number of scapular wool plait at birth（P0.01）,crimp number of scapular wool plaits at weaning（P0.01）.The individuals with genotype QQSS also had a significantly higher crimp number of rump wool plait at weaning than individuals with genotype PPRR（P0.05）.However,as for Tan sheep,the individuals with genotype QQSS only had a slightly higher least square mean than individuals with genotype PPRR or PQRS on the above traits（P0.05）.These data preliminarily indicate QQSS is the most favorable genotype affecting length and crimp number of wool plait.These variations provide opportunities for further investigating the effects of DSG4 on wool traits in sheep.

关 键 词：滩羊 DSG4基因 SNP 羊毛性状 

分 类 号：S826[农业科学—畜牧学] Q343.15[农业科学—畜牧兽医]