中药复方对夏季肉牛的影响:Ⅱ.血气指标、血清代谢产物浓度及免疫和抗氧化功能参数  

Effect of Chinese Medicine Prescription on Beef Cattle in Summer:Ⅱ.The Blood Gas Analysis,the Concentration of Metabolites and the Parameters of the Immune and Antioxidant Capability in the Serum

作　　者：汪水平[1,2] 王文娟[1] 左福元[1] 周沛[1] 赵建军[1] 张家骅[2] 

机构地区：[1]西南大学荣昌校区,重庆402460 [2]西南大学重庆市牧草与草食家畜重点实验室,重庆400716

出　　处：《畜牧兽医学报》2011年第5期734-741,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：中央高校基本科研业务费专项资金(XDJK2009C161);重庆市重大科技专项"草食畜牧良种繁育与健康养殖"(CSTC;2008AA10202008AA1019);西南大学科研基金资助项目(08BSr0908BSr11)

摘　　要：通过测定血气指标、血清代谢产物浓度及免疫和抗氧化功能参数,进一步探讨中药复方缓解夏季肉牛热应激的作用机理。选择18头月龄相近、体型结构相似、体质量300kg左右的健康西门塔尔与本地黄牛杂交的二代公牛,采用单因子完全随机试验设计,将18头牛随机分为3个组,1个对照组和2个处理组,每组6个重复,每个重复1头牛。对照组饲喂基础日粮,2个处理组分别在基础日粮水平上给每头牛日喂0.2kg复方Ⅰ和复方Ⅱ(由藿香、苍术、黄柏和石膏等中药按不同比例组成)。试验以三峡库区夏季湿热环境为热应激源,时间为2009年7月1日-9月8日,其中预饲期为10d,正式期为59d。结果表明,2种复方均能提高肉牛全血二氧化碳与氧分压、氧饱和度、二氧化碳总量、实际碳酸氢盐浓度、剩余碱与缓冲碱浓度及钾、钠、氯等离子浓度,降低全血pH、阴离子间隙、血红蛋白浓度和红细胞压积;2种复方均能降低肉牛血清尿素氮、尿酸、肌酐、甘油三酯、总胆固醇、低密度和高密度脂蛋白胆固醇、乳酸等浓度,提高血清葡萄糖浓度;2种复方均能提高肉牛血清总蛋白、白蛋白、球蛋白、C-反应蛋白、免疫球蛋白A/G/M、补体3/4、一氧化氮等浓度及白细胞介素-2水平和一氧化氮合成酶活性;2种复方均能增强肉牛血清总抗氧化能力和羟自由基抑制能力,提高血清过氧化氢酶、谷胱甘肽过氧化物酶和总超氧化物歧化酶等的活性,降低血清丙二醛浓度。研究结果为应用中药复方缓解肉牛热应激提供了科学依据。The aim of the study was to illustrate further the mechanism of action by which Chinese medicine prescription relieved the heat stress which exposed to the beef cattle in the summer by the blood gas analysis and by the determination of the concentration of metabolites and the parameters of the immune and antioxidant capability in the serum.Eighteen healthy cattle with the similar age,conformation and weight（~300 kg）,which crossbred by Simmental bull and native cow were randomly allotted into three groups.One group was the control group,and the others were the treating groups.There were 6 cattle in each group with 6 replicates and 1 cattle were used in per replicate.The experiment was designed with single factor random arrangement.The cattle in the control group were fed the basal diet.And the cattle in the two treating groups were fed the basal diets with different Chinese medicine prescriptions supplied with 0.2 kg per day per cattle,such as prescriptionⅠand prescriptionⅡ,which composed of Herba Agastachis,Rhizoma Atractylodis,Cortex Phellodendri and Gypsum Fibrosunm with different proportions,as a kind of concentrate additive,respectively.The heat stress of the cattle could be caused by the moist heat environment in the Three-gorge Reservoir District in the summer.The start and end dates of the experiment were from 1 July to 8 September in the 2009.The duration of the preliminary experiment was 10 days and the duration of the formal experiment was 59 days.The results showed that： every prescription could increase the partial pressure of CO2 and O2,saturation O2,and the concentrations of total CO2,actual bicarbonate,buffer excess,buffer base,K＋,Na＋ and Cl-,and decrease pH,hematocrit,and the concentrations of anion gap and hemoglobin in the whole blood;every prescription could decrease the blood serum concentrations of urea nitrogen,uric acid,crea,triglyceride,total cholesterol,low-density and high-density lipoprotein cholesterol and lactic acid except for the glucose concentration;every prescriptio

关 键 词：中药复方 热应激 肉牛 血液参数 

分 类 号：S853.7[农业科学—临床兽医学] S823.92[农业科学—兽医学]