低氧暴露对大鼠外周血T淋巴细胞活化的影响  

Effect of hypoxia on activation of the peripheral blood T lymphocyte in rats

作　　者：田云梅[1] 聂鸿靖[1] 刘嘉瀛[1] 张延坤[1] 张东祥[1] 汪海[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所军事环境医学研究中心,天津300050

出　　处：《中国应用生理学杂志》2011年第2期145-148,共4页Chinese Journal of Applied Physiology

基　　金：军队医药卫生杰出人才基金项目(06J023)

摘　　要：目的:探讨低氧暴露后大鼠外周血T淋巴细胞亚群及活化共刺激分子的变化,为干预措施的研究提供科学依据。方法:采用三色免疫荧光标记流式细胞仪分析法,观察在低氧暴露前和模拟海拔8 000 m低氧暴露8 h、3d6、d和10 d后,大鼠外周血T淋巴细胞亚群和T淋巴细胞活化共刺激分子的变化。结果:模拟海拔8 000 m低氧暴露8 h后,大鼠CD3+、CD8+、CD8+CD28-细胞数较低氧前均显著降低(P均<0.01);低氧暴露3 d后,除上述变化外,CD4+CD28+细胞数也显著降低(P<0.01),CD4+CD28-细胞数显著增加(P<0.01);低氧暴露6 d和10 d后,CD3+、CD4+呈现进一步降低趋势,CD8+CD28+细胞数显著增加(P<0.01)。结论:说明模拟海拔8 000 m低氧暴露8 h和3 d后,大鼠外周血CD8+、CD4+T淋巴细胞活化水平均显著下降,随着低氧暴露时间的延长,CD8+T淋巴细胞活化水平由降低变为增加。Objective：To explore the effect of hypoxia on the peripheral blood T lymphocyte subsets and co-stimulatory molecules in rats so as to provide the basis for studying the intervention measure.Methods：Before hypoxia and during hypoxia at 8 000 m for 8 h,3 d,6 d and 10 d the change of peripheral blood T lymphocyte subsets and co-stimulatory molecules in rats were detected by flowcytometer with three-color immunofluorescence label.Results：Rats were exposed to hypoxia at 8 000 m for 8 hours,and CD3＋,CD8＋,CD8＋CD28-lymphocyte percentages were significantly decreased（P0.01） compared with that before hypoxia.After 3 days of hypoxia,besides aforesaid change,CD4＋CD28＋ lymphocyte percentage also prominently decreased（P0.01） and CD4＋CD28-prominently increased（P0.01）.After 6 and 10 days of hypoxia,CD3＋、CD4＋ lymphocyte percentages were further decreased,while CD8＋CD28＋ lymphocyte percentage significantly increased（P0.01）.Conclusion：After exposed to hypoxia at 8 000 m for 8 hours and 3 days,activation of CD8＋ and CD4＋ T lymphocyte was prominently decreased,while with the prolong of exposed time activation of CD8＋ T lymphocyte was significantly increased.

关 键 词：高原低氧 免疫损伤 流式细胞术 T细胞活化 

分 类 号：R852.11[医药卫生—航空、航天与航海医学] R392[医药卫生—临床医学]