丹参注射液对庆大霉素耳中毒豚鼠耳蜗NOS异构体表达的影响  

Effects of salvia miltiorrhiza injection on gentamicin-induced expression of nitric oxide synthase isoforms in guinea pig cochlea

作　　者：王爱梅[1] 汤浩[2] 宝东艳[1] 于利[1] 

机构地区：[1]辽宁医学院生理学教研室,锦州121001 [2]中国医科大学生理学教研室,辽宁沈阳110001

出　　处：《中国应用生理学杂志》2011年第2期246-249,共4页Chinese Journal of Applied Physiology

基　　金：辽宁省博士科研启动基金(20031086)

摘　　要：目的:研究丹参注射液(SM)对庆大霉素(GM)耳中毒豚鼠耳蜗一氧化氮合酶(NOS)异构体表达的影响,探讨SM对GM耳毒性的防护机制。方法:40只豚鼠随机分成对照组、GM组、SM组和GM+SM组,应用SABC免疫组织化学方法及显微图像分析技术,观察NOS三型异构体在豚鼠耳蜗的表达;同时结合听脑干反应(ABR)测试,观察用药前后豚鼠听阈的变化。结果:GM+SM组豚鼠耳蜗诱导型NOS(iNOS/NOSⅡ)表达和ABR阈值均明显低于GM组(P<0.01);且iNOS表达变化与ABR阈值改变高度相关(|r|>0.7,P<0.01);而各组豚鼠耳蜗神经元型NOS(nNOS/NOSⅠ)和内皮型NOS(eNOS/NOSⅢ)表达均无显著性差异。结论:SM对GM耳中毒后豚鼠耳蜗nNOS和eNOS表达无影响,但可通过抑制GM所致iNOS高表达,以减少NO的过量生成,从而对GM的耳毒性损伤发挥防护作用。Objective：To investigate the effects of salvia miltiorrhiza injection（SM） on gentamicin（GM）-induced expression of nitric o-xide synthase（NOS） isoforms in guinea pig cochlea,and to explore the protective mechanism of SM on GM-induced ototoxicity.Methods：40 guinea pigs were randomly assigned to 4 groups：control group,GM group,SM group and GM plus SM group.Expression of NOS isoforms in the guinea pig cochlea was detected by the SABC method of immunohistochemistry and microscope image analysis technique.Auditory threshold was tested by auditory brainstem response（ABR） measurement.Results：Inducible NOS（iNOS/NOSⅡ） expression and ABR threshold in GM plus SM group were both significantly declined as compared with those in GM group（P0.01）.Moreover,change of iNOS expression was in high correlation with that of ABR threshold（｜r｜0.7,P0.01）.While expression of neuronal NOS（nNOS/NOSⅠ） and endothelial NOS（eNOS / NOSⅢ） showed no significant differences in all groups.Conclusion：SM had no effect on the expression of nNOS and eNOS,but could inhibit iNOS high-expression induced by GM to reduce excessive generation of NO,therefore SM could protect against GM ototoxicity.

关 键 词：丹参注射液 庆大霉素 一氧化氮合酶 耳蜗 

分 类 号：R764.43[医药卫生—耳鼻咽喉科]