脂肪源干细胞移植对糖皮质激素性骨质疏松骨量影响的实验研究  

Effect of allogenic adipose-derived stem cell transplantation on bone mass in rats with glucocorticoid-induced osteoporosis

作　　者：陶晖[1] 余美春[1] 杨会营[1] 杨春[1] 曲戎梅[1] 戴景兴[1] 余磊[1] 原林[1] 

机构地区：[1]南方医科大学人体解剖学教研室暨广东省组织构建与检测重点实验室,广东广州510515

出　　处：《南方医科大学学报》2011年第5期817-821,共5页Journal of Southern Medical University

基　　金：国家重点基础研究计划(973计划)项目(2007CB512705);国家自然科学基金青年面上项目(30801464)~~

摘　　要：目的探索异体脂肪源干细胞(ADSCs)移植对糖皮质激素性骨质疏松(GIOP)大鼠骨密度和骨组织微结构的影响,为GIOP的治疗探索一种新的方法。方法雌性Wistar大鼠随机分为空白对照组(A组)、模型组(B组)、治疗对照组(C组)和细胞治疗组(D组)。利用醋酸泼尼松龙建模成功后,C和D组分别通过尾静脉移植生理盐水和ADSCs。对各组大鼠作腰椎和股骨骨密度测量以及胫骨骨组织形态计量学分析。结果与A组相比,B组腰椎和股骨骨密度、骨小梁的相对体积、厚度和数量均明显降低,骨小梁的分离度和单位骨小梁破骨细胞数显著增加(P<0.05)。ADSCs治疗后,D组中的各项指标均明显改善,并与B组比较具有统计学差异(P<0.05),亦明显优于C组。结论 ADSCs可以有效的改善大鼠GIOP,这为GIOP的治疗提供了一种新的方法,也为"筋膜学假说"提供了部分实验支持。Objective To investigate the effects of systemic transplantation of allogenic adipose-derived stem cells（ADSCs） ,the main undifferentiated cells in the supporting-storing system based on the fasciology hypothesis,on the bone mineral density（BMD） and histomorphometry in rats with glucocorticoid-induced osteoporosis（GIOP） rats,and explore a new therapeutic approach for osteoporosis.Methods Forty female adult Wistar rats were randomized equally into blank control group（A） ,model group（B） ,control treatment group（C） and treatment group（D） .In groups B,C,and D,osteoporosis was induced by injection of prednisolone（8 mg/kg） via the tail vein 3 times a week for 12 consecutive weeks.After successful establishment of osteoporosis,allogenic ADSCs（3×106/ml） were transplanted via the tail vein.The BMD at the L3-L5 levels and of the right femurs were detected,and the histomorphometry of the right tibias was analyzed in all the rats.Results After prednisolone injection,the BMD of L3-L5 vertebrae and the right femurs,the percent trabecular area,trabecular thickness and trabecular number of the right tibias were all obviously lowered while the trabecular separation and osteoclast number increased in group B as compared to those in group A（P0.05） .Four weeks after ADSC transplantation,all these indices were significantly improved in group D,showing significant differences from those in group B（P0.05） and also group C.Conclusion Transplantation of allogenic ADSCs can restore the BMD and bone histomorphometric properties of rats with GIOP,and may serve as a potential treatment for GIOP.These results also provide partial xperimental evidence supporting the hypothesis of fasciaology.

关 键 词：筋膜学 脂肪源干细胞 骨质疏松 骨组织形态计量学 

分 类 号：R580[医药卫生—内分泌]