骨髓间充质干细胞对免疫损伤卵巢的修复作用  

Effect of mesenchymal stem cell transplantation on immunological injury of the ovary in mice

作　　者：李威娜[1] 谢琪璇[1,2] 秦俊文[1,2] 黄伟[1] 叶绍恩[1] 罗峰[1] 张春雪[1] 肖銮娟[1] 

机构地区：[1]暨南大学生殖免疫研究所,广东广州510632 [2]暨南大学再生医学教育部重点实验室,广东广州510632

出　　处：《南方医科大学学报》2011年第5期825-829,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(30370541);211工程3期生物科学技术与生物医学工程建设项目~~

摘　　要：目的探讨骨髓间充质干细胞移植对免疫损伤卵巢的修复作用。方法 6～8周龄的野生型健康ICR雌性小鼠随机分为3组,每组各12只。A组不作任何处理,B、C组利用猪卵巢蛋白主动免疫建立免疫损伤卵巢模型。而后C组腹腔移植来自GFP转基因小鼠的骨髓MSCs细胞,A、B组各注射等体积的PBS。MSCs移植后按既定时间处死小鼠,利用PCR检测卵巢中GFP基因以判断MSCs是否到达卵巢;通过对卵巢组织切片HE染色以及细胞凋亡分析来检测MSCs是否对免疫损伤卵巢有修复作用及其机制。结果 PCR结果表明MSCs经腹腔移植可直接到达免疫损伤卵巢;MSCs移植可促进免疫损伤卵巢组织的修复;相应地,移植组颗粒细胞的凋亡比非移植组明显减少。结论腹腔移植MSCs可促进免疫损伤卵巢的修复,其机制可能与减少颗粒细胞凋亡有关。Objective To investigate the effect of mesenchymal stem cell（MSC） transplantation in repairing ovarian injury in mice sensitized with porcine ovarian proteins.Methods Wild-type female mice with ICR background（6-8 weeks old） were divided randomly into groups A,B and C（n=12） .In groups B and C,the mice were treated with the total protein extract from porcine ovary to induce immunological injury of the ovary,while those in group A received no treatment.MSCs-derived from GFP transgenic mice were transplanted into the mice of group C,and equal volume of PBS was injected intraperitoneally in mice of the other two groups.PCR was used to detect GFP gene in the genomic DNA of the ovaries to assess MSCs homing in the ovary,and the reparative effect of MSCs on ovarian injury was evaluated using HE staining and TUNEL analysis.Results After transplantation,the MSCs could reach the injured ovaries to promote the repair of the ovarian injury,resulting also in reduced apoptosis of the granulosa cells（GCs） in the injured ovaries.Conclusion MSCs transplantation can promote the recovery of the immunological injury of the ovary in mice,the mechanism of which may involve reduced apoptosis of the GCs.

关 键 词：骨髓间充质干细胞 卵巢 免疫损伤 修复 

分 类 号：R711.75[医药卫生—妇产科学]