转基因红花中角质细胞生长因子KGF-1的表达  

Expression of Keratinocyte Growth Factor-1 (KGF-1) in Transgenic Safflower

作　　者：江莺[1,2] 刘秀明[1,3] 马吉胜[1] 李巍[1,3] 朱海林[1,3] 杜美丽[1,3] 李海燕[1,3] 李校堃[1] 

机构地区：[1]吉林农业大学生物反应器与药物开发教育部工程研究中心,长春130118 [2]吉林农业大学中药材学院,长春130118 [3]吉林农业大学生命科学学院,长春130118

出　　处：《植物学报》2011年第3期311-318,共8页Chinese Bulletin of Botany

基　　金：863计划(No.2007AA100503);吉林省科技发展计划重点项目(No.20070922);高等学校科技创新工程重大项目培育资金项目(No.70S018)

摘　　要：通过构建重组表达质粒载体p139035S-KGF1和根癌农杆菌介导在红花(Carthamus tinctorius)中表达角质细胞生长因子(KGF-1)。从侵染到诱导生根共需要14周,转化率达0.1%。红花子叶在潮霉素筛选培养基上培养4-5周后便可获得丛生芽,再生芽移入含潮霉素的伸长生根培养基,培养4-8周可诱导生根。通过PCR、Southern blot、RT-PCR及Western blot检测证明目的基因KGF-1已经整合到红花细胞的染色体中,实现了KGF-1外源蛋白在红花中的成功表达,为开发KGF-1蛋白新的生产途径奠定了基础。The recombinant plasmid vector p139035S-KGF1 was constructed and transfected into safflower by Agrobacterium tumefaciens transfection.The period of infection to rooting was 14 weeks,and transformation efficiency was about 0.1%.Adventitious shoots were generated after cotyledons were cultured in selecting media containing hygromycin for 4-5 weeks.Rooted safflower was obtained in 4-8 weeks.KGF-1 was transformed into safflower chromosomes and expressed successfully,as determined by PCR,Southern blot,RT-PCR and Western blot analysis,which established the basis for the production of KGF-1.

关 键 词：根癌农杆菌 角质细胞生长因子 转基因红花 

分 类 号：S567.219[农业科学—中草药栽培] Q943.2[农业科学—作物学]