萝卜带柄子叶高频再生体系的建立  

Establishment of High-frequency Regeneration System from Cotyledons with Petiole in Radish

作　　者：李海萍[1] 张鲁刚[1] 张静[1] 茹磊[1] 刘学成[1] 孙希禄[1] 

机构地区：[1]西北农林科技大学园艺学院,杨陵712100

出　　处：《植物学报》2011年第3期331-337,共7页Chinese Bulletin of Botany

基　　金：国家科技支撑计划(No.2009BADB8B03-1)

摘　　要：以11份萝卜(Raphanus sativus)基因型为材料进行子叶离体培养研究,筛选出具有较高再生率的基因型进行实验,考察基因型、外植体类型、激素配比和苗龄等因素对萝卜再生的影响。结果表明:萝卜离体再生的最佳外植体为全子叶-叶柄,最适苗龄为4天,最适培养基为MS+6mg·L-16-BA+0.05mg·L-1NAA,再生率高达86.95%,再生系数为1.80。该研究为进行萝卜遗传转化实验奠定了良好基础。We studied in vitro culture regeneration from cotyledons of 11 genotypes of radish.Those genotypes,which have a high rate of regeneration,were used as the test materials for subsequent experiments of explant type,hormone ratio,and seedling age.The regenerated plantlets,which generated roots well,were transplanted into the field.The entire cotyledon with a petiole is the optimal regeneration explant.Four days of seedling age is suitable to induce adventitious shoots.The maximum frequency of shoot regeneration was 86.95% obtained from cotyledons of Z3 in the medium Murishige and Skoog ＋ 6 mg·L-1 6-BA ＋ 0.05 mg·L-1 NAA,and the regeneration coefficient reached 1.80.These results set the basis for radish genetic transformation.

关 键 词：离体再生 萝卜 6-BA TDZ 

分 类 号：S631.1[农业科学—蔬菜学]