小黑杨应拉木上下侧cDNA文库的比较分析  

Comparative analysis of two cDNA libraries from Populus simonii ×P. nigra tension wood

作　　者：张凯旋[1] 赵桂媛[1] 刘关君[1] 刘桂丰[1] 杨传平[1] 魏志刚[1] 

机构地区：[1]东北林业大学林木遗传育种与生物技术教育部重点实验室

出　　处：《北京林业大学学报》2011年第3期8-13,共6页Journal of Beijing Forestry University

基　　金：黑龙江省重点攻关课题(GAO6B301--4);国家自然科学基金项目(30571513);东北林业大学研究生论文资助项目(gram09)

摘　　要：为了研究小黑杨应拉木的形成机制和相关基因的表达情况,通过模拟重力对小黑杨茎生长产生影响后,进行了以下研究:以小黑杨茎应拉木未成熟木质部组织为材料,分别构建了弯曲茎上侧(TW)与下侧(OW)cDNA文库,共获得了6048条高质量的ESTs序列,代表了5007条单一基因,鉴定出437条可能与应拉木形成有关的ESTs。通过比较TW与OW中的ESTs发现,纤维素合成相关基因、FLA等细胞壁相关蛋白基因以及MYB等转录因子均在TW中高表达,而木质素合成相关基因在OW中表达量较高。此外,一些参与信号转导和多糖代谢的基因在TW和OW中出现不同的表达模式。To increase our understanding of the tension wood formation and relative gene expression in Populus simonii × P. nigra, we isolated the immature xylem from both tension wood （TW） and opposite wood （OW） of bent poplars after the species growth were affected by simulated gravity. Two cDNA libraries were then constructed and used to generate 6 048 expressed sequence tags （ESTs） , which represented 5 007 unique transcripts and involved 437 ESTs in tension wood formation. The EST distributions were compared between two libraries. The results showed that genes involved in cellulose biosynthesis, cell wall proteins and transcriptional factors were found and expressed at high levels in TW, while genes involved lignin biosynthesis were expressed at high levels in OW. Moreover, genes involved in signal transduction and carbohydrate metabolism were also identified and had different expression models in TW and OW.

关 键 词：小黑杨 应拉木 相对木 木质部 CDNA文库 

分 类 号：S718.46[农业科学—林学]