IFN-γ诱导HaCaT细胞ICAM-1表达的条件优化  

Optimizing on IFN-γ-induced ICAM-1 Expression in HaCaT Cells

作　　者：伍春莲[1] 

机构地区：[1]西华师范大学生命科学学院西南野生动植物资源保护教育部重点实验室,四川南充637009

出　　处：《西华师范大学学报（自然科学版）》2011年第2期137-140,共4页Journal of China West Normal University(Natural Sciences)

基　　金：四川省教育厅青年基金项目(07ZB089);西华师范大学科研启动基金资助项目(06B026)

摘　　要：细胞间黏附分子-1(ICAM-1)是白细胞和角质细胞之间相互作用的必需因子,角质细胞中ICAM-1表达的上调与多种皮肤炎症相关.本研究为了优化γ-干扰素(IFN-γ)诱导HaCaT细胞ICAM-1表达的条件,用不同浓度的IFN-γ诱导HaCaT细胞不同时间,流式细胞仪检测ICAM-1表达.结果发现选用1 000U/mL的剂量诱导HaCaT细胞24h ICAM-1的表达是最高的.从而表明IFN-γ可以上调ICAM-1的表达,为后期药物治疗皮肤炎症奠定基础.Intercellular adhesion molecule-1（ICAM-1） was necessary for leukocyte and kerationocyte interaction.Upregulation of ICAM-1 expression in keratinocytes had correlation with many skin inflammation.The purpose of this study was to optimize conditions of IFN-γ-induced ICAM-1 expression on HaCaT cells in vitro.HaCaT cells were stimulated by IFN-γ at different concentration and time respectively.IFN-γ-induced ICAM-1 expression was detected in HaCaT cells by flow cytometry.Finally treatment of HaCaT cells with 1000U/mL IFN-γfor 24h markedly induced ICAM-1 expression.The results indicated that IFN-γ could up-regulate.The expression of ICAM-1 laid the foundation for skin inflammation therapeutics by using medicine latterly.

关 键 词：HACAT细胞 Γ-干扰素 细胞间黏附分子-1 优化 

分 类 号：R751[医药卫生—皮肤病学与性病学]