高等真核细胞受极低频磁场辐照后基因差异表达的分析  

Analysis of differentially expressed genes induced by extremely low frequency magnetic field in higher eukaryote

作　　者：吴瑞英[1] 姜槐[1] 董海涛[2] 胡根林[1] 

机构地区：[1]浙江大学医学院微波研究室,杭州310006 [2]浙江大学医学院,杭州310006

出　　处：《中华劳动卫生职业病杂志》1999年第6期331-334,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金!资助( 基金号:39630100;39770633)

摘　　要：目的　从基因水平研究电磁场的非热效应及其可能的机制。方法　以高等真核细胞（ 人恶性淋巴瘤细胞） 为模型，运用ｍ ＲＮＡ 差异显示法（ｍ ＲＮＡ ｄｉｆｆｅｒｅｎｔｉａｌ ｄｉｓｐｌａｙ ，ＤＤ） 技术分析受５０ Ｈｚ 极低频磁场（ＥＦＬＭＦ） 辐照及假辐照２４ 小时后的细胞基因转录的差异。结果　用７５ 个引物组合获得１１个对ＥＬＦＭＦ 显示差异反应的ｃＤＮＡ 片段，表现为：（１） 片段只出现在受辐照的细胞，而未出现在非辐照的细胞中；（２） 片段只出现在非辐照的细胞中，而未出现在受辐照的细胞中；（３） 片段在辐照和非辐照细胞中均有表达，但表达强度有明显差异。回收这些片段，经ＰＣＲ 重扩增及反向Ｎｏｒｔｈｅｒｎ 初步筛选，结合Ｎｏｒｔｈｅｒｎ ｂｌｏｔ 鉴定，部分ｃＤＮＡ 得到证实为磁场特异反应基因。结论　低强度ＥＬＦＭＦ 可影响基因表达。Objective To clarify nonthermal effects of electromagnetic fields on biological system and its mechanism. Methods The study was designed to screen and identify differentially expressed genes following 50 Hz extremely low frequency magnetic field(ELFMF) exposure by mRNA differential display. Results Using 75 primer combinations,11 of cDNA fragments were found differentially displayed responding to MF exposure,including:cDNA fragments only present in MF exposed cultures;cDNA fragments only absent in MF exposed cultures,and cDNA fragments present in both MF and control groups with greater different extent.The interesting fragments were then recovered from the dried gel,reamplified by PCR.Some of them were identified by reverse Northern and Northern blot. Conclusion ELFMF may influence gene transcription in human cell.

关 键 词：极低频磁场 MRNA 差异显示法 基因差异片段 

分 类 号：Q354.2[生物学—遗传学] R691.5[医药卫生—泌尿科学]