同型半胱氨酸对血管抗氧化酶基因表达的影响  

Effect of Homocysteine on The Expression of Endothelial Antioxidative Enzymes in Vein

作　　者：单妍[1] 张敏华[1] 张明[1] 田兴亚[1] 

机构地区：[1]昆明医学院海源学院生物化学教研室,云南昆明650106

出　　处：《云南师范大学学报（自然科学版）》2011年第3期64-67,共4页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：昆明医学院海源学院科研基金资助项目(JYT0901H)

摘　　要：目的探讨同型半胱氨酸(Hcy)诱导抗氧化酶基因表达的影响。方法采用实时定量RT-PCR测定基因的表达,用DCF—DA荧光探针测定细胞内ROS;研究材料为HUEVC及高Hcy小鼠胸主动脉;结果同型半胱氨酸能诱导人脐静脉血管内皮细胞(HUEVC)的ROS水平升高,抑制HUEVC的谷胱甘肽过氧化物酶(GSHPx)和过氧化氢酶(CAT)基因的表达。在高同型半胱氨酸血症小鼠胸主动脉中,谷胱甘肽过氧化物酶、过氧化氢酶和锰超氧化物歧化酶(Mn-SOD)基因表达均明显降低。结论 Hcy能促进上皮细胞内活性氧的产生,可明显促进氧化损伤,同时显著降低抗氧化酶(谷胱甘肽过氧化物酶、过氧化氢酶及锰超氧化物歧化酶)的活性。To study the effects of homocysteine（Hcy） at different concentrations on the expression of antioxidative enzymes in cultured HUEVC and in aorta of mice with hyperhomocysteinemia.Methods： The expression of tested genes was detected by realtime RT-PCR.The levels of ROS in cells were measured by using DCF-DA as a fluorescence probe;Research material for the HUEVC and the aorta of mice with hyperhomocysteinemia.Results：Homocysteine promoted the levels of ROS and down-regulated the expression of catalase and glutathione peroxidase in HUEVC.The expression of glutathione peroxidase,catalase,and Mn-SOD decreased in aorta of mice with hyperhomocysteinemia.Conclusion：Hcy could significantly urge the generation of intracellular reactive oxygen species and promote oxidative injiury.At the same time Hcy could notablely inhibit the activeties of antioxidative enzymes（GSHPx、Catalase and Mn-SOD）.

关 键 词：同型半胱氨酸 血管内皮细胞 活性氧物质 抗氧化酶 

分 类 号：R113[医药卫生—公共卫生与预防医学]