凝胶层析法测定蛋白含量的方法  

A method for protein concentration determination by gel chromatography method

作　　者：王娜娜[1] 姚秀清[1] 谢小莉[1] 张全[2] 

机构地区：[1]辽宁石油化工大学环境与生物工程学院,辽宁抚顺113001 [2]抚顺石油化工研究院,辽宁抚顺113001

出　　处：《应用化工》2011年第5期906-908,共3页Applied Chemical Industry

摘　　要：利用蛋白纯化仪建立一种凝胶层析测定蛋白含量的方法,采用HiTrapTMDesalting 5×5 mL色谱柱,流动相为超纯水,以蛋白浓度为横坐标,以蛋白出峰面积为纵坐标,建立标准曲线。与BCA试剂盒法比较,BCA试剂盒法相对误差范围为9.13%～144.00%,此法的相对误差范围为0.78%～44.72%,可见凝胶层析法测定结果准确性高,操作简单,快速,是一种可行的测蛋白含量的方法。Protein was purified by protein purification device to establish a method for determination of protein content.By HiTrapTM Desalting 5×5 mL column,mobile phase was ultra-pure water with protein concentration as abscissa and peak area of protein as the vertical axis,the standard curve was established.Compared with BCA kit method,the relative error range of BCA kit method was 9.13%～144.00%,the range of relative error of protein content was 0.78%～44.72%.Measureed by protein purification device,the method is high accuracy,simple and fast.It is a feasible method of measuring protein content.

关 键 词：蛋白测定 蛋白纯化仪 凝胶层析 BCA法 

分 类 号：Q512[生物学—生物化学]