鞘内及侧脑室注射NMDA、荷包牡丹碱对异氟烷肌松作用的影响  

Effects of intrathecal and intracerebroventricular injection of NMDA or bicuculline on isoflurane-induced muscle relaxation

作　　者：刘亚君[1] 周美艳[2] 张明阳 戴体俊[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,221002 [2]徐州医学院药学院

出　　处：《江苏医药》2011年第11期1250-1252,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金(30872432);江苏省自然科学基金(BK2001143)

摘　　要：目的探讨异氟烷的肌肉松弛作用机制及与N-甲基-D-天冬氨酸(NMDA)、γ-氨基丁酸A(GABAA)受体的关系。方法小鼠腹腔注射异氟烷,2 min后在鞘内或侧脑室注射不同剂量的NMDA或荷包牡丹碱(Bic)。用YLS-13A大小鼠抓力测定仪测定各组给药前后不同时间点的抓力。结果鞘内注射102、0 ng的NMDA或鞘内注射不同剂量的Bic及侧脑室注射0.2μg的Bic均能部分拮抗异氟烷所致肌松作用(P<0.01或P<0.05)。结论异氟烷主要作用于脊髓产生肌松作用,NMDA及GABAA受体可能是异氟烷产生肌松作用的靶点之一。Objective To investigate the mechanisms of isoflurane-induced muscle relaxation and its relationship with N-methyl-D-aspartate（NMDA） and GABAA receptors.Methods Two minutes after intraperitoneal injection of isoflurane,the rats were treated with intrathecal and intracerebroventricular injection of different doses of NMDA or bicuculline（Bic）.Grip strength of the rats was measured with YLS-13A grip strength meter at different time points.Results The isoflurane-induced muscle relaxation was partly antagonized by intrathecal injection of NMDA 10ng or 20ng,intrathecal injection of different doses of Bic,or intracerebroventricular injection of Bic 0.2μg（P0.05 or P0.01）.Conclusion The isoflurane-induced muscle relaxation is mainly through its effect on the spinal cord.NMDA receptors and GABAA receptors are probably the targets of muscle relaxation produced by isoflurane.

关 键 词：异氟烷 荷包牡丹碱 N-甲基-D-天冬氨酸 Γ-氨基丁酸A受体 

分 类 号：R338[医药卫生—人体生理学]