基于改良RAPD标记的花梅与果梅品种的遗传关系分析  

Analysis of genetic relationship of flowering and fruiting mei cultivars based on modified RAPD marker

作　　者：王玉娟[1] 房经贵[1] 于华平[1] 汪诗珊 李晓颖[1] 陈翔高[1] 

机构地区：[1]南京农业大学园艺学院江苏省果树品种改良与种苗繁育工程中心,江苏南京210095 [2]南京中山陵园管理局,江苏南京210014

出　　处：《植物资源与环境学报》2011年第2期28-34,共7页Journal of Plant Resources and Environment

基　　金：国家教育部新世纪优秀人才支持计划项目(NCET-08-0796)

摘　　要：为了探讨梅（Prunus mume Sieb.et Zucc.）的2种类型———花梅与果梅的遗传多样性,利用23条长度为11 bp的随机引物以及严格筛选退火温度的改良RAPD-PCR优化体系对25个花梅和21个果梅品种的基因组总DNA进行了分析,并在此基础上采用UPGMA聚类方法对46个花梅与果梅品种间的遗传关系进行了分析。结果显示：使用重复性好、条带清晰且多态性强的23条随机引物,共从46个花梅和果梅品种基因组总DNA中扩增出131条带,其中多态性条带107条,多态性条带百分率达81.7%,说明花梅和果梅各品种间具有丰富的遗传多样性。聚类分析结果显示：46个花梅和果梅品种的遗传相似系数为0.63~0.83;在遗传相似系数0.66处,供试的46个品种被分为5组。A组包含6个果梅品种和11个花梅品种;B组仅包含4个花梅品种;C组包含16个品种,其中15个品种为果梅;D组仅包含4个花梅品种;E组也仅包含5个花梅品种。研究结果表明：花梅和果梅具有相近的亲缘关系,遗传背景相似。In order to explore the genetic diversity of two types of Prunus mume Sieb.et Zucc.,which are flowering and fruiting mei,total genomic DNA from 25 cultivars of flowering mei and 21 cultivars of fruiting mei was analyzed by modified RAPD-PCR optimized system with strict screening of anneal temperature and using 23 random primers with the length of 11 bp.And on this basis,the genetic relationship of 46 cultivars of flowering and fruiting mei were analyzed by UPGMA cluster method.The results show that 131 bands are amplified from total genomic DNA of 46 cultivars by using of 23 random primers with good reproducibility,clear bands and rich polymorphism,in which there are 107 polymorphic bands and the percentage of polymorphic bands accounts for 81.7%.It means that there is rich genetic diversity among different cultivars of flowering and fruiting mei.The result of cluster analysis shows that the genetic similarity coefficient of 46 cultivars of flowering and fruiting mei is 0.63-0.83 and at the 0.66 point of genetic similarity coefficient,the 46 cultivars are divided into 5 groups.In which,Group A contains 6 cultivars of fruiting mei and 11 cultivars of flowering mei;Group B only contains 4 cultivars of flowering mei;Group C contains 16 cultivars in which 15 cultivars are fruiting mei;Group D only contains 4 cultivars of flowering mei;Group E also only contains 5 cultivars of flowering mei.It is suggested that flowering and fruiting mei have close relationship and similar genetic background.

关 键 词：改良RAPD标记 花梅 果梅 遗传多样性 遗传关系 聚类分析 

分 类 号：Q946-33[生物学—植物学] S662.4[农业科学—果树学]