口腔链球菌对白假丝酵母菌的拮抗作用  

The antagonistic effect of the oral Streptococcus on the Saccharomyces albicans in vitro

作　　者：张琳[1] 孙珺[1] 李多[1] 肖晓蓉[1] 朱硃[1] 龚其美[1] 周红梅[2] 

机构地区：[1]口腔疾病研究国家重点实验室四川大学 [2]四川大学华西口腔医院黏膜科,成都610041

出　　处：《华西口腔医学杂志》2011年第3期302-305,共4页West China Journal of Stomatology

基　　金：高等学校博士学科点专项科研基金资助项目(20070610-067)

摘　　要：目的观测3种口腔链球菌对白假丝酵母菌的生长有无抑制作用。方法采用直接点种法、反点种菌落计数法和液体共培养法观测口腔变异链球菌、血链球菌、唾液链球菌对白假丝酵母菌的拮抗作用。结果 1)直接点种法中各组均未观察到清晰的抑菌圈;2)唾液链球菌软琼脂上的白假丝酵母菌菌落数较对照组减少(P<0.05);3)活菌与白假丝酵母菌液体共培养中,在各个时间点上变异链球菌组与对照组之间菌落计数差异均有统计学意义(P=0.001);4)细菌培养基上清液与白假丝酵母菌液体共培养中,在24、48、72h时间点上,各实验组与对照组之间菌落计数差异均有统计学意义(P=0.001);多数时间点上,各实验组间菌落计数差异无统计学意义(P>0.05)。结论 3种口腔链球菌对白假丝酵母菌的体外生长有明显抑制作用,但尚不能确定哪种链球菌的抑制作用更强,并且抑制作用会逐渐衰减。Objective To investigate the antagonistic effects of three species of oral Streptococcus on the growth of oral Saccharomyces albicans in vitro.Methods Direct inoculation method,reverse inoculation method and mixed culture methods were respectively chosen to observe the changes of Saccharomyces albicans colony formation on the effects of Streptococcus mutans,Streptococcus sanguis and Streptococcus salivarius.Results 1）No clear inhibition zone was ob-served in each of the groups by direct inoculation method.2）Compared with the control groups,Saccharomyces albicans colony formation on soft agar of Streptococcus sanguis decreased significantly（P0.05）.3）Mixed culture method results showed that Streptococcus mutans could inhibit the growth of Saccharomyces albicans significantly at different time points（P=0.001）.4）Under the action of bacteria culture supernatant,the count of Saccharomyces albicans in experiment groups showed statistical significance when compared with the control groups at 24,48,72 h（P=0.001）;The differences among the experimental groups were of no statistical significance at majority times（P0.05）.Conclusion Streptococcus mutans,Streptococcus sanguis,and Streptococcus salivarius could obviously inhibit the growth of Saccharomyces albicans in vitro.However,it is still unclear that among which the inhibition effects is stronger.The antagonistic effects is wea-kened gradually.

关 键 词：白假丝酵母菌 变异链球菌 唾液链球菌 血链球菌 拮抗作用 

分 类 号：R711.3[医药卫生—妇产科学]