非完全互补shRNA对RNA聚合酶Ⅱ启动子调控的RNAi的影响  

RNA Interference Induced by Not-fully Complementary shRNA Under the Control of an RNA Polymerase Ⅱ Promoter

作　　者：丁晶晶[1] 任雪玲[1] 宋雨[1] 张丹丹[1] 张振中[1] 张云[1] 

机构地区：[1]郑州大学药学院,郑州450001

出　　处：《中国生物化学与分子生物学报》2011年第6期568-573,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.30772543)资助项目~~

摘　　要：RNA干扰(RNAi)是一种转录后基因沉默技术,可有效诱导序列特异性基因沉默.由RNA聚合酶Ⅱ启动子调控表达的小发卡RNA可有效介导RNAi效应,为组织特异性基因沉默提供了一条新的途径.但是,由RNA聚合酶Ⅱ启动子调控表达的小发卡RNA(shRNA)在序列上与靶基因非完全互补对RNAi效应的影响鲜有报道.本文初步探索RNA聚合酶Ⅱ启动子调控表达的shRNA碱基发生突变或缺失对RNAi效应的影响.研究表明,靶向hTERT mRNA的碱基突变shRNA显著降低RNAi效应,而靶向GFP mRNA的碱基缺失shRNA对RNAi效应没有显著影响.本研究为非完全互补shRNA对RNAi效应的进一步深入研究提供了理论与实验依据.RNA interference（RNAi） is a powerful tool to effectively silence gene expression in a sequence-specific manner at the post-transcriptional level.Small hairpin RNAs（shRNAs） under the control of an RNA polymerase Ⅱ promoter provide an alternative approach to efficiently induce tissue-specific RNA interference.However,little is known about whether not-fully complementary shRNA from the RNA polymerase Ⅱ promoter induce RNA interference.In this study,we observed mutation-shRNA targeting GFP could induce RNAi efficiently,while deletion-shRNA targeting hTERT couldn＇t.This result provides further evidence of the potential effects of not-fully complementary shRNA.

关 键 词：小发卡RNA RNA干扰 RNA聚合酶Ⅱ启动子 突变 缺失 

分 类 号：Q753[生物学—分子生物学]