脾虚大鼠胰岛胰多肽免疫阳性物质分布变化  

The Changes of the Immunoreactive Area Distribution and mRNA Expression of Pancreatic Polypeptide in the Pancreas in Spleen Deficiency Rats

作　　者：覃尧[1] 周露云[1] 高月异[1] 刘发强[1] 张挪威[1] 朱晓宇[1] 滕可导[1] 许剑琴[1] 

机构地区：[1]中国农业大学动物医学院,北京100193

出　　处：《畜牧兽医学报》2011年第6期865-869,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：高等学校博士学科点专项科研基金(20100008110024);中国农业大学URP项目"脾虚大鼠基因芯片中与代谢相关基因差异表达的聚类分析";国家自然科学基金(30771591);国家"十一五"科技支撑计划(2008BADB4B01;2008BADB4B07)

摘　　要：脾虚证的临床表现以消化系统功能障碍为主,其中胰腺外分泌功能减退明显,且伴有食欲与体质量的显著下降。胰多肽(PP)是胰岛周边F细胞分泌的主要胃肠激素,对胰腺外分泌功能与食欲的调节有抑制作用,可导致氧化代谢增强,引起体质量下降。本试验旨在揭示大鼠胰岛胰多肽分泌变化与脾虚证发病之间的关系。笔者以利血平脾虚大鼠模型为研究对象,采用SP染色进行免疫组织化学研究,观察大鼠胰岛中胰多肽免疫阳性物质的分布情况。脾虚组与对照组的大鼠胰岛中胰多肽免疫阳性物质面积与胰岛面积百分比相比,胰头部分未见明显差异(P>0.05);但脾虚组胰中、胰尾的PP免疫阳性物质所占面积极显著大于对照组(P<0.01)。结果表明,脾虚证大鼠胰岛中胰多肽总分泌量极显著增加。因此,胰多肽的分泌增加是导致脾虚证动物出现胰腺外分泌功能低下、食欲减退以及体质量下降的主要因素。The spleen deficiency syndrome plays a significant role in the concept of Chinese traditional medicine.Its primary clinical manifestation is the functional impairment of digestive system,showing pancreatic external secretion declined,food intake reduced and weight lose.On the other hand,the pancreatic polypeptide（PP）,released principally from released from pancreatic islet F-cells,has well-proved function including the inhibition of pancreatic secretion,relaxation of gallbladder,increase oxygen consumption appetite regulation.In the present study we aim at find out the relationship between the islet PP secretory activity and the syndrome using reserpine-induced spleen deficiency Wistar rats.Immunohistochemical SP method was used to observe the distribution characteristics of PP immune in pancreatic islets.Compared with the controls,both the immunoreactive area distribution of PP in the pancreas of the spleen deficiency rats showed remarkably significant increase（P0.01）.The results suggested that PP is one of the critical factors contributing to spleen deficiency syndrome in rats.

关 键 词：脾虚证 胰多肽 胰岛 WISTAR大鼠 免疫阳性物质 SP染色 免疫组织化学 

分 类 号：S853[农业科学—临床兽医学]